skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Long-term expression of human adenosine deaminase in vascular smooth muscle cells of rats: A model for gene therapy

Abstract

Gene transfer into vascular smooth muscle cells in animals was examined by using recombinant retroviral vectors containing an Escherichia coli {beta}-galactosidase gene or a human adenosine deaminase gene. Direct gene transfer by infusion of virus into rat carotid arteries was not observed. However, gene transfer by infection of smooth muscle cells in culture and seeding of the transduced cells onto arteries that had been denuded of endothelial cells was successful. Potentially therapeutic levels of human adenosine deaminase activity were detected over 6 months of observation, indicating the utility of vascular smooth muscle cells for gene therapy in humans.

Authors:
;  [1]; ; ;  [2]
  1. Fred Hutchinson Cancer Research Center, Seattle, WA (United States)
  2. Univ. of Washington, Seattle (United States)
Publication Date:
OSTI Identifier:
5559487
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (United States)
Additional Journal Information:
Journal Volume: 89:3; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ENDOTHELIUM; GENE RECOMBINATION; NON-PEPTIDE C-N HYDROLASES; GENE REGULATION; ARTERIES; ENZYME IMMUNOASSAY; MUSCLES; RATS; THERAPY; ANIMAL TISSUES; ANIMALS; BIOASSAY; BLOOD VESSELS; BODY; CARDIOVASCULAR SYSTEM; ENZYMES; HYDROLASES; IMMUNOASSAY; MAMMALS; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RODENTS; TISSUES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Lynch, C M, Miller, A D, Clowes, M M, Osborne, W R.A., and Clowes, A W. Long-term expression of human adenosine deaminase in vascular smooth muscle cells of rats: A model for gene therapy. United States: N. p., 1992. Web. doi:10.1073/pnas.89.3.1138.
Lynch, C M, Miller, A D, Clowes, M M, Osborne, W R.A., & Clowes, A W. Long-term expression of human adenosine deaminase in vascular smooth muscle cells of rats: A model for gene therapy. United States. https://doi.org/10.1073/pnas.89.3.1138
Lynch, C M, Miller, A D, Clowes, M M, Osborne, W R.A., and Clowes, A W. 1992. "Long-term expression of human adenosine deaminase in vascular smooth muscle cells of rats: A model for gene therapy". United States. https://doi.org/10.1073/pnas.89.3.1138.
@article{osti_5559487,
title = {Long-term expression of human adenosine deaminase in vascular smooth muscle cells of rats: A model for gene therapy},
author = {Lynch, C M and Miller, A D and Clowes, M M and Osborne, W R.A. and Clowes, A W},
abstractNote = {Gene transfer into vascular smooth muscle cells in animals was examined by using recombinant retroviral vectors containing an Escherichia coli {beta}-galactosidase gene or a human adenosine deaminase gene. Direct gene transfer by infusion of virus into rat carotid arteries was not observed. However, gene transfer by infection of smooth muscle cells in culture and seeding of the transduced cells onto arteries that had been denuded of endothelial cells was successful. Potentially therapeutic levels of human adenosine deaminase activity were detected over 6 months of observation, indicating the utility of vascular smooth muscle cells for gene therapy in humans.},
doi = {10.1073/pnas.89.3.1138},
url = {https://www.osti.gov/biblio/5559487}, journal = {Proceedings of the National Academy of Sciences of the United States of America; (United States)},
issn = {0027-8424},
number = ,
volume = 89:3,
place = {United States},
year = {Sat Feb 01 00:00:00 EST 1992},
month = {Sat Feb 01 00:00:00 EST 1992}
}