Carbon-13 nuclear magnetic resonance spectroscopy of lipids: Differential line broadening due to cross-correlation effects as a probe of membrane structure

Oldfield, E; Adebodun, F; Chung, J; Montez, B; Park, Ki Deok; Le, Hongbiao; Phillips, B

doi:10.1021/bi00110a003

Title: Carbon-13 nuclear magnetic resonance spectroscopy of lipids: Differential line broadening due to cross-correlation effects as a probe of membrane structure

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Abstract

The authors have obtained proton-coupled carbon-13 nuclear magnetic resonance (NMR) spectra of a variety of lipid-water and lipid-drug-water systems, at 11.7 T, as a function of temperature, using the 'magic-angle' sample-spinning (MAS) NMR technique. The resulting spectra show a wide range of line shapes, due to interferences between dipole-dipole and dipole-chemical shielding anisotropy interactions. The differential line-broadening effects observed are particularly large for aromatic and olefinic (sp{sup 2}) carbon atom sites. Coupled spectra of the tricyclic antidepressants desipramine and imipramine, in 1,2-dimyristoyl-sn-glycero-3-phosphocholine-water mesophases, show well-resolved doublets having different line shapes for each of the four aromatic methine groups, due to selective averaging of the four C-H dipolar interactions due to rapid motion about the director (or drug C{sub 2}) axis. {sup 2}H NMR spectra of (2,4,6,8-{sup 2}H{sub 4})desipramine (and imipramine) in the same 1,2-dimyristoyl-sn-glycero-3-phosphocholine-water mesophase exhibit quadrupole splittings of {approximately}0-2 and {approximately}20 kHz, indicating an approximate magic-angle orientation of the C2-{sup 2}H({sup 1}H) and C8-{sup 2}H({sup 1}H) vectors with respect to an axis of motional averaging, in accord with the {sup 13}C NMR results. The good qualitative agreement between {sup 13}C and {sup 2}H NMR results suggests that useful orientational ({sup 2}H NMR like) information can be deduced from natural-abundancemore »« less

Authors:

Oldfield, E; Adebodun, F; Chung, J; Montez, B; Park, Ki Deok; Le, Hongbiao; Phillips, B ^[1]

Univ. of Illinois, Urbana (United States)

Publication Date:: Tue Nov 19 00:00:00 EST 1991

OSTI Identifier:: 5559189

Resource Type:: Journal Article

Journal Name:: Biochemistry; (United States)

Additional Journal Information:: Journal Volume: 30:46; Journal ID: ISSN 0006-2960

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; ANTIDEPRESSANTS; BIOLOGICAL EFFECTS; CELL MEMBRANES; MOLECULAR STRUCTURE; LIPIDS; NUCLEAR MAGNETIC RESONANCE; CARBON 13; DEUTERIUM COMPOUNDS; PHOSPHOLIPIDS; PROTONS; BARYONS; CARBON ISOTOPES; CELL CONSTITUENTS; CENTRAL NERVOUS SYSTEM AGENTS; DRUGS; ELEMENTARY PARTICLES; ESTERS; EVEN-ODD NUCLEI; FERMIONS; HADRONS; HYDROGEN COMPOUNDS; ISOTOPES; LIGHT NUCLEI; MAGNETIC RESONANCE; MEMBRANES; NUCLEI; NUCLEONS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; PSYCHOTROPIC DRUGS; RESONANCE; STABLE ISOTOPES; 550201* - Biochemistry- Tracer Techniques

Citation Formats


                    Oldfield, E, Adebodun, F, Chung, J, Montez, B, Park, Ki Deok, Le, Hongbiao, and Phillips, B. Carbon-13 nuclear magnetic resonance spectroscopy of lipids: Differential line broadening due to cross-correlation effects as a probe of membrane structure.  United States: N. p., 1991. 
        Web.  doi:10.1021/bi00110a003.

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                    Oldfield, E, Adebodun, F, Chung, J, Montez, B, Park, Ki Deok, Le, Hongbiao, & Phillips, B. Carbon-13 nuclear magnetic resonance spectroscopy of lipids: Differential line broadening due to cross-correlation effects as a probe of membrane structure.  United States.  https://doi.org/10.1021/bi00110a003

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                    Oldfield, E, Adebodun, F, Chung, J, Montez, B, Park, Ki Deok, Le, Hongbiao, and Phillips, B. 1991.  
        "Carbon-13 nuclear magnetic resonance spectroscopy of lipids: Differential line broadening due to cross-correlation effects as a probe of membrane structure".  United States.  https://doi.org/10.1021/bi00110a003.

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@article{osti_5559189,

  title        = {Carbon-13 nuclear magnetic resonance spectroscopy of lipids: Differential line broadening due to cross-correlation effects as a probe of membrane structure},

  author       = {Oldfield, E and Adebodun, F and Chung, J and Montez, B and Park, Ki Deok and Le, Hongbiao and Phillips, B},

  abstractNote = {The authors have obtained proton-coupled carbon-13 nuclear magnetic resonance (NMR) spectra of a variety of lipid-water and lipid-drug-water systems, at 11.7 T, as a function of temperature, using the 'magic-angle' sample-spinning (MAS) NMR technique. The resulting spectra show a wide range of line shapes, due to interferences between dipole-dipole and dipole-chemical shielding anisotropy interactions. The differential line-broadening effects observed are particularly large for aromatic and olefinic (sp{sup 2}) carbon atom sites. Coupled spectra of the tricyclic antidepressants desipramine and imipramine, in 1,2-dimyristoyl-sn-glycero-3-phosphocholine-water mesophases, show well-resolved doublets having different line shapes for each of the four aromatic methine groups, due to selective averaging of the four C-H dipolar interactions due to rapid motion about the director (or drug C{sub 2}) axis. {sup 2}H NMR spectra of (2,4,6,8-{sup 2}H{sub 4})desipramine (and imipramine) in the same 1,2-dimyristoyl-sn-glycero-3-phosphocholine-water mesophase exhibit quadrupole splittings of {approximately}0-2 and {approximately}20 kHz, indicating an approximate magic-angle orientation of the C2-{sup 2}H({sup 1}H) and C8-{sup 2}H({sup 1}H) vectors with respect to an axis of motional averaging, in accord with the {sup 13}C NMR results. The good qualitative agreement between {sup 13}C and {sup 2}H NMR results suggests that useful orientational ({sup 2}H NMR like) information can be deduced from natural-abundance {sup 13}C NMR spectra of a variety of mobile solids.},

  doi          = {10.1021/bi00110a003},

  url          = {https://www.osti.gov/biblio/5559189},
  journal      = {Biochemistry; (United States)},

  issn         = {0006-2960},

  number       = ,

  volume       = 30:46,

  place        = {United States},

  year         = {Tue Nov 19 00:00:00 EST 1991},

  month        = {Tue Nov 19 00:00:00 EST 1991}

}

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