Effects of experiences on synaptic protein phosphorylation in vitro
In vitro transfer of /sup 32/P from (gamma-/sup 32/P)-ATP into proteins of particulate fractions from osmotically shocked preparations enriched in rat brain synaptosomes was studied. Phosphate incorporation into protein bands of apparent molecular weights (MW) 44,000, 24,000, 21,000, and 19,000 was affected by the prior experiences of the rats from which the particulate fractions were prepared. Incorporation into all four proteins was increased in particulate fraction from previously naive rats that received active avoidance training. Handling of the subjects prior to training prevented the response of the 24,000 MW protein to training. Phosphate incorporation into 24,000 and 19,000 MW proteins was increased in preparations from previously naive rats that underwent a yoked experience, while incorporation into the 21,000 MW protein was slightly decreased. The yoked experience did not affect in vitro phosphate incorporation into any of these proteins in particulate fractions from previously handled rats.
- Research Organization:
- Department of Biochemistry and Nutrition, University of North Carolina, Chapel Hill
- OSTI ID:
- 5552155
- Journal Information:
- Pharmacol., Biochem. Behav.; (United States), Vol. 18:6
- Country of Publication:
- United States
- Language:
- English
Similar Records
Prostaglandin F2 alpha administered in vivo induces Ca2+-dependent protein phosphorylation in rat luteal tissue
Phosphorylation of a high molecular weight DNA polymerase. cap alpha
Related Subjects
LEARNING
BIOCHEMISTRY
PROTEINS
PHOSPHORYLATION
ATP
CELL MEMBRANES
NERVE CELLS
PHOSPHORUS 32
TRACER TECHNIQUES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHEMISTRY
DAYS LIVING RADIOISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LIGHT NUCLEI
MEMBRANES
NUCLEI
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
SOMATIC CELLS
550201* - Biochemistry- Tracer Techniques
550101 - Behavioral Biology- Tracer Techniques