Metal binding components in human amniotic fluid

Paterson, P G; Zlotkin, S H; Sarkar, B

Title: Metal binding components in human amniotic fluid

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Abstract

Amniotic fluid is a potential source of both nutritionally essential and toxic metals for the fetus. As the binding pattern of these metals in amniotic fluid may be one of the determining factors in their availability to the fetus, the objective of this study was to investigate metal binding in vitro. The binding of six trace metals, Mn(II), Ni(II), Zn(II), Cu(II), Cd(II), and Fe(III), to components of human amniotic fluid was studied by Sephadex G-100 gel filtration at physiological pH, using radioisotopes as tracers and 50 mM TRIS/HCl as the elution buffer. The amniotic fluid was collected at 16-16.5 weeks gestation by amniocentesis and pooled for analysis. Extensive amounts of Fe, Cu, Zn, and Cd and small amounts of Mn and Ni were bound to high molecular weight proteins with elution patterns similar to those seen for the binding of these metals in serum. In addition, large amounts of Fe, Mn, Ni and Cd and small amounts of Zn and Cu were associated with low molecular weight component(s). The identity of these latter components is unknown, but they play an important biological role in amniotic fluid.

Authors:

Paterson, P G; Zlotkin, S H; Sarkar, B ^[1]

Hospital for Sick Children, Toronto, Ontario (Canada)

Publication Date:: Mon Feb 26 00:00:00 EST 1990

OSTI Identifier:: 5551531

Report Number(s):: CONF-9004153-
Journal ID: ISSN 0892-6638; CODEN: FAJOE

Resource Type:: Conference

Journal Name:: FASEB Journal (Federation of American Societies for Experimental Biology); (United States)

Additional Journal Information:: Journal Volume: 4:3; Conference: 74. annual meeting of the Federation of American Societies for Experimental Biology, Washington, DC (United States), 1-5 Apr 1990; Journal ID: ISSN 0892-6638

Country of Publication:: United States

Language:: English

Subject:: 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; AMNIOTIC FLUID; CHEMICAL COMPOSITION; METALS; BIOLOGICAL AVAILABILITY; CADMIUM; COPPER; FETUSES; IN VITRO; IRON; MANGANESE; NICKEL; ZINC; BIOLOGICAL MATERIALS; BODY FLUIDS; ELEMENTS; MATERIALS; TRANSITION ELEMENTS; 560300* - Chemicals Metabolism & Toxicology

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                    Paterson, P G, Zlotkin, S H, and Sarkar, B. Metal binding components in human amniotic fluid.  United States: N. p., 1990. 
        Web.

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                    Paterson, P G, Zlotkin, S H, & Sarkar, B. Metal binding components in human amniotic fluid.  United States.

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                    Paterson, P G, Zlotkin, S H, and Sarkar, B. 1990.  
        "Metal binding components in human amniotic fluid".  United States.

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@article{osti_5551531,

  title        = {Metal binding components in human amniotic fluid},

  author       = {Paterson, P G and Zlotkin, S H and Sarkar, B},

  abstractNote = {Amniotic fluid is a potential source of both nutritionally essential and toxic metals for the fetus. As the binding pattern of these metals in amniotic fluid may be one of the determining factors in their availability to the fetus, the objective of this study was to investigate metal binding in vitro. The binding of six trace metals, Mn(II), Ni(II), Zn(II), Cu(II), Cd(II), and Fe(III), to components of human amniotic fluid was studied by Sephadex G-100 gel filtration at physiological pH, using radioisotopes as tracers and 50 mM TRIS/HCl as the elution buffer. The amniotic fluid was collected at 16-16.5 weeks gestation by amniocentesis and pooled for analysis. Extensive amounts of Fe, Cu, Zn, and Cd and small amounts of Mn and Ni were bound to high molecular weight proteins with elution patterns similar to those seen for the binding of these metals in serum. In addition, large amounts of Fe, Mn, Ni and Cd and small amounts of Zn and Cu were associated with low molecular weight component(s). The identity of these latter components is unknown, but they play an important biological role in amniotic fluid.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/5551531},
  journal      = {FASEB Journal (Federation of American Societies for Experimental Biology); (United States)},

  issn         = {0892-6638},

  number       = ,

  volume       = 4:3,

  place        = {United States},

  year         = {Mon Feb 26 00:00:00 EST 1990},

  month        = {Mon Feb 26 00:00:00 EST 1990}

}

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