Presence of dopamine D-2 receptors in human tumoral cell lines

Journal Article · Mon Jul 31 00:00:00 EDT 1989 · Biochemical and Biophysical Research Communications; (USA)

DOI:https://doi.org/10.1016/0006-291X(89)92349-8· OSTI ID:5547392

Sokoloff, P; Riou, J F; Martres, M P; Schwartz, J C ^[1]

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1. Centre Paul Broca, Paris (France)

({sup 125}I) Iodosulpride binding was examined on eight human cell lines derived from lung, breast and digestive tract carcinomas, neuroblastomas and leukemia. Specific binding was detected in five of these cell lines. In the richest cell line N417, derived from small cell lung carcinoma, ({sup 125}I) iodosulpride bound with a high affinity (Kd = 1.3 nM) to an apparently homogeneous population of binding site (Bmax = 1,606 sites per cell). These sites displayed a typical D-2 specificity, established with several dopaminergic agonists and antagonists selective of either D-1 or D-2 receptor subtypes. In addition, dopamine, apomorphine and RU 24926 distinguished high- and low-affinity sites, suggesting that the binding sites are associated with a G-protein. The biological significance and the possible diagnostic implication of the presence of D-2 receptors on these cell lines are discussed.

• OSTI ID: 5547392
• Journal Information: Biochemical and Biophysical Research Communications; (USA), Vol. 162:2; ISSN 0006-291X
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
DOPAMINE
RECEPTORS
BIOCHEMICAL REACTION KINETICS
CELL MEMBRANES
DIGESTIVE SYSTEM
IODINE 125
LEUKEMIA
LUNGS
MAMMARY GLANDS
MAN
MORPHINE
NEOPLASMS
TRACER TECHNIQUES
TUMOR CELLS
ALKALOIDS
AMINES
ANALGESICS
ANIMAL CELLS
ANIMALS
AROMATICS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BODY
CARDIOTONICS
CARDIOVASCULAR AGENTS
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DAYS LIVING RADIOISOTOPES
DISEASES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
GLANDS
HEMIC DISEASES
HYDROXY COMPOUNDS
IMMUNE SYSTEM DISEASES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
NARCOTICS
NEUROREGULATORS
NUCLEI
ODD-EVEN NUCLEI
OPIUM
ORGANIC COMPOUNDS
ORGANS
PHENOLS
POLYPHENOLS
PRIMATES
PROTEINS
RADIOISOTOPES
REACTION KINETICS
RESPIRATORY SYSTEM
SYMPATHOMIMETICS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques