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Title: Regeneration of bovine and octopus opsins in situ with natural and artificial retinals

The authors consider the problem of color regulation in visual pigments for both bovine rhodopsin and octopus rhodopsin. Both pigments have 11-cis-retinal as their chromophore. These rhodopsins were bleached in their native membranes, and the opsins were regenerated with natural and artificial chromophores. Both bovine and octopus opsins were regenerated with the 9-cis- and 11-cis-retinal isomers, but the octopus opsin was additionally regenerated with the 13-cis and all-trans isomers. Titration of the octopus opsin with 11-cis-retinal gave an extinction coefficient for octopus rhodopsin of 27,000 {plus minus} 3,000 M{sup {minus}1} cm{sup {minus}1} at 475 nm. The absorption maxima of bovine artificial pigments formed by regenerating opsin with the 11-cis dihydro series of chromophores support a color regulation model for bovine rhodopsin in which the chromophore-binding site of the protein has two negative charges: one directly hydrogen bonded to the Schiff base nitrogen and another near carbon-13. Formation of octopus artificial pigments with both all-trans and 11-cis dihydro chromophores leads to a similar model for octopus rhodopsin and metarhodopsin: there are two negative charges in the chromophore-binding site, one directly hydrogen bonded to the Schiff base nitrogen and a second near carbon-13. The interaction of this second charge with the chromophoremore » in octopus rhodopsin is weaker than in bovine, while in metarhodopsin it is as strong as in bovine.« less
Authors:
; ; ; ; ; ; ; ; ; ;  [1]
  1. (Univ. of Illinois, Urbana-Champaign (USA))
Publication Date:
OSTI Identifier:
5547144
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochemistry; (USA); Journal Volume: 28:6
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; RHODOPSIN; MOLECULAR STRUCTURE; VITAMIN A; ABSORPTION SPECTRA; CARBON 13; CATTLE; CHEMICAL BONDS; LIQUID COLUMN CHROMATOGRAPHY; MEMBRANES; MOLLUSCS; VISION; ANIMALS; AQUATIC ORGANISMS; CARBON ISOTOPES; CHROMATOGRAPHY; DOMESTIC ANIMALS; EVEN-ODD NUCLEI; INVERTEBRATES; ISOTOPES; LIGHT NUCLEI; MAMMALS; NUCLEI; ORGANIC COMPOUNDS; PIGMENTS; PROTEINS; RUMINANTS; SEPARATION PROCESSES; SPECTRA; STABLE ISOTOPES; VERTEBRATES; VITAMINS 550201* -- Biochemistry-- Tracer Techniques