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Title: Characterization of deoxyribonucleic acid from cells infected with Aleutian disease virus

Abstract

Viral DNA was extracted from Crandell feline kidney (CRFK) cells infected with Aleutian disease virus (ADV) and labeled with (/sup 3/H)thymidine. The sedimentation coefficient in alkaline sucrose gradients was 16S corresponding to a molecular weight of 1.5 X 10(6). The buoyant densities of DNA from infected and control cells were determined by isopyknic sedimentation in CsCl and NaI gradients. Two additional peaks of (/sup 3/H)DNA were found in infected cells, but not in control cell extracts. Fractionation of this DNA on hydroxylapatite indicated that the new peaks represented a single-stranded component, density 1.728 g/cm3, and a double-stranded component, presumed to be a viral replicative intermediate, density 1.718 g/cm3. The target antigen formation in CRFK cells was measured by gamma-irradiation of ADV and assayed for focus formation. The calculated size of ADV based on these measurements was 1.1 X 10(6). The H-1 parvovirus also was shown to have a size of 1.5 X 10(6) daltons for both antigen and plaque formation. The data indicated similarities existed between ADV and other autonomously replicating parvoviruses in most properties, except that less-than-unit length genome of ADV may be transcribed.

Authors:
; ;
Publication Date:
Research Org.:
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Illinois, Urbana
OSTI Identifier:
5546866
Resource Type:
Journal Article
Journal Name:
Am. J. Vet. Res.; (United States)
Additional Journal Information:
Journal Volume: 44:7
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; DNA; BIOSYNTHESIS; CENTRIFUGATION; LABELLING; MOLECULAR WEIGHT; SIZE; VIRUSES; COMPARATIVE EVALUATIONS; ANTIGENS; CATS; CELL CULTURES; ELECTROPHORESIS; GAMMA RADIATION; LIQUID COLUMN CHROMATOGRAPHY; THYMIDINE; TRACER TECHNIQUES; TRITIUM COMPOUNDS; ANIMALS; AZINES; CHROMATOGRAPHY; ELECTROMAGNETIC RADIATION; HETEROCYCLIC COMPOUNDS; IONIZING RADIATIONS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; MICROORGANISMS; NUCLEIC ACIDS; NUCLEOSIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PARASITES; PYRIMIDINES; RADIATIONS; RIBOSIDES; SEPARATION PROCESSES; SYNTHESIS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Hahn, E C, Ramos, L, and Kenyon, A J. Characterization of deoxyribonucleic acid from cells infected with Aleutian disease virus. United States: N. p., 1983. Web.
Hahn, E C, Ramos, L, & Kenyon, A J. Characterization of deoxyribonucleic acid from cells infected with Aleutian disease virus. United States.
Hahn, E C, Ramos, L, and Kenyon, A J. 1983. "Characterization of deoxyribonucleic acid from cells infected with Aleutian disease virus". United States.
@article{osti_5546866,
title = {Characterization of deoxyribonucleic acid from cells infected with Aleutian disease virus},
author = {Hahn, E C and Ramos, L and Kenyon, A J},
abstractNote = {Viral DNA was extracted from Crandell feline kidney (CRFK) cells infected with Aleutian disease virus (ADV) and labeled with (/sup 3/H)thymidine. The sedimentation coefficient in alkaline sucrose gradients was 16S corresponding to a molecular weight of 1.5 X 10(6). The buoyant densities of DNA from infected and control cells were determined by isopyknic sedimentation in CsCl and NaI gradients. Two additional peaks of (/sup 3/H)DNA were found in infected cells, but not in control cell extracts. Fractionation of this DNA on hydroxylapatite indicated that the new peaks represented a single-stranded component, density 1.728 g/cm3, and a double-stranded component, presumed to be a viral replicative intermediate, density 1.718 g/cm3. The target antigen formation in CRFK cells was measured by gamma-irradiation of ADV and assayed for focus formation. The calculated size of ADV based on these measurements was 1.1 X 10(6). The H-1 parvovirus also was shown to have a size of 1.5 X 10(6) daltons for both antigen and plaque formation. The data indicated similarities existed between ADV and other autonomously replicating parvoviruses in most properties, except that less-than-unit length genome of ADV may be transcribed.},
doi = {},
url = {https://www.osti.gov/biblio/5546866}, journal = {Am. J. Vet. Res.; (United States)},
number = ,
volume = 44:7,
place = {United States},
year = {Fri Jul 01 00:00:00 EDT 1983},
month = {Fri Jul 01 00:00:00 EDT 1983}
}