Studies of DNA and chromosome damage in skin fibroblasts and blood lymphocytes from psoriasis patients treated with 8-methoxypsoralen and UVA irradiation
Exposure of human lymphocytes and skin fibroblasts in vitro to a single, clinically used dose of PUVA, i.e., 0.1 micrograms/ml of 8-methoxypsoralen (8-MOP) plus 0.9-4 J/cm2 of longwave ultraviolet radiation (UVA), lead to the formation of DNA damage as determined by alkaline elution, and to chromosome aberrations and sister chromatid exchanges (SCE). When lymphocyte-enriched plasma was obtained from psoriasis patients 2 h after oral intake of 8-MOP and then UVA irradiated (1.8-3.6 J/cm2) in vitro, an increased frequency of chromosome aberrations and SCE was observed. Normal levels of chromosome aberrations and SCE were found in lymphocytes of psoriasis patients after 3-30 weeks of PUVA treatment in vivo. A small but statistically significant increase in the SCE frequency was observed in the lymphocytes of psoriasis patients treated for 1-6 years with PUVA (mean 18.0 SCE/cell) as compared with before PUVA (mean 15.8, p less than 0.05). Skin fibroblasts of psoriasis patients analyzed 5 years after the start of PUVA treatment showed a normal number of SCE but a high fraction of filter-retained DNA in the alkaline elution assay, suggesting the presence of cross-linked DNA.
- Research Organization:
- Department of Clinical Genetics, Karolinska Hospital, Stockholm, Sweden
- OSTI ID:
- 5545718
- Journal Information:
- J. Invest. Dermatol.; (United States), Vol. 81:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
BIOLOGICAL RADIATION EFFECTS
PSORALEN
SYNERGISM
ULTRAVIOLET RADIATION
CHROMOSOMAL ABERRATIONS
FIBROBLASTS
IN VITRO
LYMPHOCYTES
MAN
PSORIASIS
SISTER CHROMATID EXCHANGES
SKIN
ANIMAL CELLS
ANIMALS
ANTICOAGULANTS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CONNECTIVE TISSUE CELLS
COUMARINS
DISEASES
DRUGS
ELECTROMAGNETIC RADIATION
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
LEUKOCYTES
MAMMALS
MATERIALS
MUTATIONS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PRIMATES
RADIATION EFFECTS
RADIATIONS
SKIN DISEASES
SOMATIC CELLS
VERTEBRATES
560114* - Radiation Effects on Biochemicals- In Animals- (-1987)