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Title: Turnover of cytokeratin polypeptides in mouse hepatocytes

Abstract

The turnover of cytokeratin polypeptides A (equivalent to No. 8 of the human cytokeratin catalog) and D (equivalent to human cytokeratin No. 18) of mouse hepatocytes was studied by pulse-labeling of mouse liver proteins after intraperitoneal injection of L-(guanido{sup 14}C)arginine and ({sup 14}C)sodium bicarbonate. With L-(guanido-{sup 14}C)arginine a rapid increase in the specific radioactivity of both cytokeratins was observed which reached a plateau between 12 and 24 h. With ({sup 14}C)sodium bicarbonate maximal specific radioactivity was obtained at 6 h followed by a rapid decrease to half maximum values within the subsequent 6 h and then a slower decrease. Half-lives were determined from the decrease of specific radioactivities after pulse-labeling by least-squares plots and found to be 84 h (for cytokeratin component A) and 104 h (component D) for arginine labeling . Values obtained after bicarbonate labeling were similar (95 h for A and 98 h for D). These results show that liver cytokeratins are relatively stable proteins and suggest that components A and D are synthesized and degraded at similar rates, probably in a coordinate way.

Authors:
; ;  [1];  [2]
  1. Univ. of Graz (Austria)
  2. German Cancer Research Center, Heidelberg (West Germany)
Publication Date:
OSTI Identifier:
5534025
Resource Type:
Journal Article
Journal Name:
Experimental Cell Research; (United States)
Additional Journal Information:
Journal Volume: 173:1; Journal ID: ISSN 0014-4827
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; KERATIN; METABOLISM; POLYPEPTIDES; BIOSYNTHESIS; ACID CARBONATES; ARGININE; CARBON 14 COMPOUNDS; LIVER CELLS; MICE; TRACER TECHNIQUES; AMINO ACIDS; ANIMAL CELLS; ANIMALS; CARBON COMPOUNDS; CARBOXYLIC ACIDS; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PEPTIDES; PROTEINS; RODENTS; SCLEROPROTEINS; SOMATIC CELLS; SYNTHESIS; VERTEBRATES; 550301* - Cytology- Tracer Techniques

Citation Formats

Denk, H, Lackinger, E, Zatloukal, K, and Franke, W W. Turnover of cytokeratin polypeptides in mouse hepatocytes. United States: N. p., 1987. Web. doi:10.1016/0014-4827(87)90339-9.
Denk, H, Lackinger, E, Zatloukal, K, & Franke, W W. Turnover of cytokeratin polypeptides in mouse hepatocytes. United States. https://doi.org/10.1016/0014-4827(87)90339-9
Denk, H, Lackinger, E, Zatloukal, K, and Franke, W W. 1987. "Turnover of cytokeratin polypeptides in mouse hepatocytes". United States. https://doi.org/10.1016/0014-4827(87)90339-9.
@article{osti_5534025,
title = {Turnover of cytokeratin polypeptides in mouse hepatocytes},
author = {Denk, H and Lackinger, E and Zatloukal, K and Franke, W W},
abstractNote = {The turnover of cytokeratin polypeptides A (equivalent to No. 8 of the human cytokeratin catalog) and D (equivalent to human cytokeratin No. 18) of mouse hepatocytes was studied by pulse-labeling of mouse liver proteins after intraperitoneal injection of L-(guanido{sup 14}C)arginine and ({sup 14}C)sodium bicarbonate. With L-(guanido-{sup 14}C)arginine a rapid increase in the specific radioactivity of both cytokeratins was observed which reached a plateau between 12 and 24 h. With ({sup 14}C)sodium bicarbonate maximal specific radioactivity was obtained at 6 h followed by a rapid decrease to half maximum values within the subsequent 6 h and then a slower decrease. Half-lives were determined from the decrease of specific radioactivities after pulse-labeling by least-squares plots and found to be 84 h (for cytokeratin component A) and 104 h (component D) for arginine labeling . Values obtained after bicarbonate labeling were similar (95 h for A and 98 h for D). These results show that liver cytokeratins are relatively stable proteins and suggest that components A and D are synthesized and degraded at similar rates, probably in a coordinate way.},
doi = {10.1016/0014-4827(87)90339-9},
url = {https://www.osti.gov/biblio/5534025}, journal = {Experimental Cell Research; (United States)},
issn = {0014-4827},
number = ,
volume = 173:1,
place = {United States},
year = {Sun Nov 01 00:00:00 EST 1987},
month = {Sun Nov 01 00:00:00 EST 1987}
}