Inhibitory effects of tetradecanoylphorbol acetate and diacylglycerol on erythropoietin production in human renal carcinoma cell cultures

Journal Article · Sun Nov 01 00:00:00 EST 1987 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(87)90338-7· OSTI ID:5533424

Hagiwara, Masamichi; Nagakura, Kazuhiko; Ueno, Munehisa; Fisher, J W ^[1]

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1. Tulane Univ., New Orleans, LA (United States)

A human renal carcinoma from a patient with an erythrocytosis, serially transplanted into athymic nude mice, was grown in primary monolayer cell cultures. After reaching confluency the cultured cells formed multicellular hemicysts (domes) which became more abundant as the cultures approached saturation density. Erythropoietin (Ep) production by this renal carcinoma in culture was only slightly increased at the time of semiconfluency but showed a marked increase in Ep levels in the culture medium after the cultures reached confluency, in parallel with an increase in dome formation. The phorbol ester tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA) showed a significant dose-related inhibitory effect on Ep production and dome formation in the renal carcinoma cell cultures, suggesting an important role of protein kinase C, the only known receptor for TPA, in inhibiting the expression of differentiated phenotypes in the renal carcinoma cells. These studies suggest a role of the inositol-lipid second messenger path and protein kinase C in the regulation of Ep production.

• OSTI ID: 5533424
• Journal Information: Experimental Cell Research; (United States), Vol. 173:1; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ERYTHROPOIETIN
RADIOIMMUNOASSAY
PHORBOL ESTERS
BIOLOGICAL EFFECTS
ACETATES
CARCINOMAS
ENZYME ACTIVITY
INHIBITION
KIDNEYS
PHOSPHOTRANSFERASES
TUMOR CELLS
ANIMAL CELLS
BIOASSAY
BODY
CARBOXYLIC ACID SALTS
CARCINOGENS
DIAGNOSTIC TECHNIQUES
DISEASES
ENZYMES
ESTERS
HORMONES
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
MITOGENS
NEOPLASMS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PHOSPHORUS-GROUP TRANSFERASES
PROTEINS
RADIOASSAY
RADIOIMMUNODETECTION
RADIOIMMUNOLOGY
TRACER TECHNIQUES
TRANSFERASES
550601* - Medicine- Unsealed Radionuclides in Diagnostics