Comparison of hprt variant frequencies and chromosome aberration frequencies in lymphocytes from radiotherapy and chemotherapy patients: A prospective study
Abstract
The autoradiographic 6-thioguanine-resistant mutant lymphocyte assay and a chromosome aberration assay were used to determine the time-course of appearance and persistence of elevated frequencies of hprt variants and dicentric chromosomes in patients receiving x-irradiation therapy. The hprt mutation assays were done with frozen/thawed lymphocytes isolated from aliquots of the same blood samples used for the chromosome aberration assays. Five multiple sclerosis patients were also studied before and at 2 and 4 wk intervals after treatment with monthly i.v. doses of 750 mg/m{sup 2} of cyclophosphamide (CP). There were no significant elevations in chromosome aberrations at these post-treatment sample times. The results demonstrate the complementary nature of these two human monitoring assays and emphasize the importance of careful selection of optimal sampling times.
- Authors:
-
- Univ. of Texas Medical Branch, Galveston (United States)
- Publication Date:
- OSTI Identifier:
- 5530644
- Resource Type:
- Journal Article
- Journal Name:
- Environmental and Molecular Mutagenesis; (United States)
- Additional Journal Information:
- Journal Volume: 18:2; Journal ID: ISSN 0893-6692
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CHEMOTHERAPY; SIDE EFFECTS; CHROMOSOMAL ABERRATIONS; MUTATION FREQUENCY; RADIOTHERAPY; AUTORADIOGRAPHY; BLADDER; DICENTRIC CHROMOSOMES; ENDOXAN; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; LUNGS; LYMPHOCYTES; NEOPLASMS; PATIENTS; PROSTATE; UROGENITAL SYSTEM DISEASES; UTERUS; X RADIATION; ALKYLATING AGENTS; ANIMAL CELLS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CHROMOSOMES; CONNECTIVE TISSUE CELLS; DISEASES; DRUGS; ELECTROMAGNETIC RADIATION; ENZYMES; FEMALE GENITALS; GLANDS; GLYCOSYL TRANSFERASES; IMMUNOSUPPRESSIVE DRUGS; IONIZING RADIATIONS; LEUKOCYTES; MALE GENITALS; MATERIALS; MEDICINE; MUTATIONS; NUCLEAR MEDICINE; ORGANIC COMPOUNDS; ORGANS; PENTOSYL TRANSFERASES; PROTEINS; RADIATIONS; RADIOLOGY; RESPIRATORY SYSTEM; SOMATIC CELLS; THERAPY; TRANSFERASES; URINARY TRACT; 560151* - Radiation Effects on Animals- Man; 560300 - Chemicals Metabolism & Toxicology
Citation Formats
Ammenheuser, M M, Au, W W, Whorton, Jr, E B, Belli, J A, and Ward, Jr, J B. Comparison of hprt variant frequencies and chromosome aberration frequencies in lymphocytes from radiotherapy and chemotherapy patients: A prospective study. United States: N. p., 1991.
Web. doi:10.1002/em.2850180208.
Ammenheuser, M M, Au, W W, Whorton, Jr, E B, Belli, J A, & Ward, Jr, J B. Comparison of hprt variant frequencies and chromosome aberration frequencies in lymphocytes from radiotherapy and chemotherapy patients: A prospective study. United States. https://doi.org/10.1002/em.2850180208
Ammenheuser, M M, Au, W W, Whorton, Jr, E B, Belli, J A, and Ward, Jr, J B. 1991.
"Comparison of hprt variant frequencies and chromosome aberration frequencies in lymphocytes from radiotherapy and chemotherapy patients: A prospective study". United States. https://doi.org/10.1002/em.2850180208.
@article{osti_5530644,
title = {Comparison of hprt variant frequencies and chromosome aberration frequencies in lymphocytes from radiotherapy and chemotherapy patients: A prospective study},
author = {Ammenheuser, M M and Au, W W and Whorton, Jr, E B and Belli, J A and Ward, Jr, J B},
abstractNote = {The autoradiographic 6-thioguanine-resistant mutant lymphocyte assay and a chromosome aberration assay were used to determine the time-course of appearance and persistence of elevated frequencies of hprt variants and dicentric chromosomes in patients receiving x-irradiation therapy. The hprt mutation assays were done with frozen/thawed lymphocytes isolated from aliquots of the same blood samples used for the chromosome aberration assays. Five multiple sclerosis patients were also studied before and at 2 and 4 wk intervals after treatment with monthly i.v. doses of 750 mg/m{sup 2} of cyclophosphamide (CP). There were no significant elevations in chromosome aberrations at these post-treatment sample times. The results demonstrate the complementary nature of these two human monitoring assays and emphasize the importance of careful selection of optimal sampling times.},
doi = {10.1002/em.2850180208},
url = {https://www.osti.gov/biblio/5530644},
journal = {Environmental and Molecular Mutagenesis; (United States)},
issn = {0893-6692},
number = ,
volume = 18:2,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 1991},
month = {Tue Jan 01 00:00:00 EST 1991}
}