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Title: Comparison of hprt variant frequencies and chromosome aberration frequencies in lymphocytes from radiotherapy and chemotherapy patients: A prospective study

Abstract

The autoradiographic 6-thioguanine-resistant mutant lymphocyte assay and a chromosome aberration assay were used to determine the time-course of appearance and persistence of elevated frequencies of hprt variants and dicentric chromosomes in patients receiving x-irradiation therapy. The hprt mutation assays were done with frozen/thawed lymphocytes isolated from aliquots of the same blood samples used for the chromosome aberration assays. Five multiple sclerosis patients were also studied before and at 2 and 4 wk intervals after treatment with monthly i.v. doses of 750 mg/m{sup 2} of cyclophosphamide (CP). There were no significant elevations in chromosome aberrations at these post-treatment sample times. The results demonstrate the complementary nature of these two human monitoring assays and emphasize the importance of careful selection of optimal sampling times.

Authors:
; ; ; ;  [1]
  1. Univ. of Texas Medical Branch, Galveston (United States)
Publication Date:
OSTI Identifier:
5530644
Resource Type:
Journal Article
Journal Name:
Environmental and Molecular Mutagenesis; (United States)
Additional Journal Information:
Journal Volume: 18:2; Journal ID: ISSN 0893-6692
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CHEMOTHERAPY; SIDE EFFECTS; CHROMOSOMAL ABERRATIONS; MUTATION FREQUENCY; RADIOTHERAPY; AUTORADIOGRAPHY; BLADDER; DICENTRIC CHROMOSOMES; ENDOXAN; HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE; LUNGS; LYMPHOCYTES; NEOPLASMS; PATIENTS; PROSTATE; UROGENITAL SYSTEM DISEASES; UTERUS; X RADIATION; ALKYLATING AGENTS; ANIMAL CELLS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CHROMOSOMES; CONNECTIVE TISSUE CELLS; DISEASES; DRUGS; ELECTROMAGNETIC RADIATION; ENZYMES; FEMALE GENITALS; GLANDS; GLYCOSYL TRANSFERASES; IMMUNOSUPPRESSIVE DRUGS; IONIZING RADIATIONS; LEUKOCYTES; MALE GENITALS; MATERIALS; MEDICINE; MUTATIONS; NUCLEAR MEDICINE; ORGANIC COMPOUNDS; ORGANS; PENTOSYL TRANSFERASES; PROTEINS; RADIATIONS; RADIOLOGY; RESPIRATORY SYSTEM; SOMATIC CELLS; THERAPY; TRANSFERASES; URINARY TRACT; 560151* - Radiation Effects on Animals- Man; 560300 - Chemicals Metabolism & Toxicology

Citation Formats

Ammenheuser, M M, Au, W W, Whorton, Jr, E B, Belli, J A, and Ward, Jr, J B. Comparison of hprt variant frequencies and chromosome aberration frequencies in lymphocytes from radiotherapy and chemotherapy patients: A prospective study. United States: N. p., 1991. Web. doi:10.1002/em.2850180208.
Ammenheuser, M M, Au, W W, Whorton, Jr, E B, Belli, J A, & Ward, Jr, J B. Comparison of hprt variant frequencies and chromosome aberration frequencies in lymphocytes from radiotherapy and chemotherapy patients: A prospective study. United States. https://doi.org/10.1002/em.2850180208
Ammenheuser, M M, Au, W W, Whorton, Jr, E B, Belli, J A, and Ward, Jr, J B. 1991. "Comparison of hprt variant frequencies and chromosome aberration frequencies in lymphocytes from radiotherapy and chemotherapy patients: A prospective study". United States. https://doi.org/10.1002/em.2850180208.
@article{osti_5530644,
title = {Comparison of hprt variant frequencies and chromosome aberration frequencies in lymphocytes from radiotherapy and chemotherapy patients: A prospective study},
author = {Ammenheuser, M M and Au, W W and Whorton, Jr, E B and Belli, J A and Ward, Jr, J B},
abstractNote = {The autoradiographic 6-thioguanine-resistant mutant lymphocyte assay and a chromosome aberration assay were used to determine the time-course of appearance and persistence of elevated frequencies of hprt variants and dicentric chromosomes in patients receiving x-irradiation therapy. The hprt mutation assays were done with frozen/thawed lymphocytes isolated from aliquots of the same blood samples used for the chromosome aberration assays. Five multiple sclerosis patients were also studied before and at 2 and 4 wk intervals after treatment with monthly i.v. doses of 750 mg/m{sup 2} of cyclophosphamide (CP). There were no significant elevations in chromosome aberrations at these post-treatment sample times. The results demonstrate the complementary nature of these two human monitoring assays and emphasize the importance of careful selection of optimal sampling times.},
doi = {10.1002/em.2850180208},
url = {https://www.osti.gov/biblio/5530644}, journal = {Environmental and Molecular Mutagenesis; (United States)},
issn = {0893-6692},
number = ,
volume = 18:2,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 1991},
month = {Tue Jan 01 00:00:00 EST 1991}
}