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Title: Repair of gamma-ray-induced DNA base damage in xeroderma pigmentosum cells

Abstract

The repair of DNA damage produced by /sup 137/Cs gamma irradiation was measured with a preparation from Micrococcus luteus containing DNA damage-specific endonucleases in combination with alkaline elution. The frequency of these endonuclease sensitive sites (ESS) was determined after 54 or 110 Gy of oxic irradiation in normal and xeroderma pigmentosum (XP) fibroblasts from complementation groups A, C, D, and G. Repair was rapid in all cell strains with greater than 50% repair after 1.5 h of repair incubation. At later repair times, 12-17 h, more ESS remained in XP than in normal cells. The frequency of excess ESS in XP cells was approximately 0.04 per 10(9) Da of DNA per Gy which was equivalent to 10% of the initial ESS produced. The removal of ESS was comparable in XP cells with normal radiosensitivity and XP3BR cells which have been reported to be moderately radiosensitive.

Authors:
; ;
Publication Date:
Research Org.:
National Cancer Institute, Bethesda, MD
OSTI Identifier:
5528916
Resource Type:
Journal Article
Journal Name:
Radiat. Res.; (United States)
Additional Journal Information:
Journal Volume: 1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; FIBROBLASTS; GENETIC RADIATION EFFECTS; XP CELLS; DNA REPAIR; RADIOSENSITIVITY; CELL CULTURES; CESIUM 137; DNA; DOSE-RESPONSE RELATIONSHIPS; ENDONUCLEASES; GAMMA RADIATION; MICROCOCCUS; XERODERMA PIGMENTOSUM; ALKALI METAL ISOTOPES; ANIMAL CELLS; BACTERIA; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; CESIUM ISOTOPES; CONNECTIVE TISSUE CELLS; DISEASES; DNA-ASE; ELECTROMAGNETIC RADIATION; ENZYMES; ESTERASES; GENETIC EFFECTS; HYDROLASES; IONIZING RADIATIONS; ISOTOPES; MICROORGANISMS; NUCLEI; NUCLEIC ACIDS; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; PHOSPHODIESTERASES; RADIATION EFFECTS; RADIATIONS; RADIOISOTOPES; RECOVERY; REPAIR; SKIN DISEASES; SOMATIC CELLS; YEARS LIVING RADIOISOTOPES; 560121* - Radiation Effects on Cells- External Source- (-1987)

Citation Formats

Fornace, Jr, A J, Dobson, P P, and Kinsella, T J. Repair of gamma-ray-induced DNA base damage in xeroderma pigmentosum cells. United States: N. p., 1986. Web. doi:10.2307/3576562.
Fornace, Jr, A J, Dobson, P P, & Kinsella, T J. Repair of gamma-ray-induced DNA base damage in xeroderma pigmentosum cells. United States. https://doi.org/10.2307/3576562
Fornace, Jr, A J, Dobson, P P, and Kinsella, T J. 1986. "Repair of gamma-ray-induced DNA base damage in xeroderma pigmentosum cells". United States. https://doi.org/10.2307/3576562.
@article{osti_5528916,
title = {Repair of gamma-ray-induced DNA base damage in xeroderma pigmentosum cells},
author = {Fornace, Jr, A J and Dobson, P P and Kinsella, T J},
abstractNote = {The repair of DNA damage produced by /sup 137/Cs gamma irradiation was measured with a preparation from Micrococcus luteus containing DNA damage-specific endonucleases in combination with alkaline elution. The frequency of these endonuclease sensitive sites (ESS) was determined after 54 or 110 Gy of oxic irradiation in normal and xeroderma pigmentosum (XP) fibroblasts from complementation groups A, C, D, and G. Repair was rapid in all cell strains with greater than 50% repair after 1.5 h of repair incubation. At later repair times, 12-17 h, more ESS remained in XP than in normal cells. The frequency of excess ESS in XP cells was approximately 0.04 per 10(9) Da of DNA per Gy which was equivalent to 10% of the initial ESS produced. The removal of ESS was comparable in XP cells with normal radiosensitivity and XP3BR cells which have been reported to be moderately radiosensitive.},
doi = {10.2307/3576562},
url = {https://www.osti.gov/biblio/5528916}, journal = {Radiat. Res.; (United States)},
number = ,
volume = 1,
place = {United States},
year = {Tue Apr 01 00:00:00 EST 1986},
month = {Tue Apr 01 00:00:00 EST 1986}
}