Passive transfer with serum and IgG antibodies of irradiated cercaria-induced resistance against Schistosoma mansoni in mice
The role of humoral immunity to Schistosoma mansoni infection in C57BL/6J mice was examined by employing a passive transfer system. Sera from highly resistant mice that had been exposed to two or three immunizations with 50-kilorad-gamma-irradiated cercariae were tested for their ability to transfer protection against S. mansoni challenge. All five batches of serum tested were observed to have protective activity. Immune serum recipients exhibited statistically significant reductions in challenge worm burdens of 20 to 50% compared with recipients of normal serum or no serum. The most consistent level of resistance was obtained when immune serum was administered several days post-challenge, i.e., at a time coincident with schistosomulum residence in the lungs. Furthermore, it was shown that the protective activity in immune serum was associated with factors that bind to staphylococcal protein A and that are precipitated by 50% ammonium sulfate; thus it appears that the protective factors in immune serum are IgG antibodies.
- Research Organization:
- Naval Medical Research Institute, Bethesda, MD
- OSTI ID:
- 5528751
- Journal Information:
- J. Immunol.; (United States), Vol. 7
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
SCHISTOSOMA
IMMUNE SERUMS
ANTIBODIES
BIOLOGICAL RADIATION EFFECTS
GAMMA RADIATION
IMMUNE REACTIONS
IMMUNITY
IMMUNOGLOBULINS
INFECTIVITY
MICE
VACCINES
ANIMALS
BIOLOGICAL EFFECTS
ELECTROMAGNETIC RADIATION
GLOBULINS
HELMINTHS
IONIZING RADIATIONS
MAMMALS
ORGANIC COMPOUNDS
PLATYHELMINTHS
PROTEINS
RADIATION EFFECTS
RADIATIONS
RODENTS
TREMATODES
VERTEBRATES
560134* - Radiation Effects on Microorganisms- Vaccine Preparation & Other Applications- (-1987)