Desaturation and chain elongation of essential fatty acids in isolated liver cells from rat and rainbow trout

Journal Article · Sat Mar 01 00:00:00 EST 1986 · Lipids; (United States)

DOI:https://doi.org/10.1007/BF02534822· OSTI ID:5528529

Hagve, T A; Christophersen, B O; Dannevig, B H

Isolated hepatocytes from rainbow trout and rat were incubated with /sup 14/C-labeled linoleic acid, linolenic acid, dihomogammalinolenic acid or eicosapentaenoic acid. The most striking difference in the desaturase activity was the lower level of delta 5 desaturase in trout than in rat. No delta 4 desaturation of 22:4(n-6) to 22:5(n-6) was observed in either of the two species, while the conversion of 22:5(n-3) to 22:6(n-3) was significant in both groups and highest in rainbow trout. The chain-elongating activity was remarkably similar in the two species, except for the dead-end elongation which was distinctly more important in fish.

• Research Organization: Univ. of Oslo, Rikshospitalet, Norway
• OSTI ID: 5528529
• Journal Information: Lipids; (United States), Vol. 3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
EICOSANOIC ACID
LABELLING
LINOLEIC ACID
OXYGENASES
ENZYME ACTIVITY
BIOCHEMICAL REACTION KINETICS
CARBON 14 COMPOUNDS
COMPARATIVE EVALUATIONS
LIVER CELLS
PHOSPHOLIPIDS
RATS
TROUT
ANIMAL CELLS
ANIMALS
AQUATIC ORGANISMS
CARBOXYLIC ACIDS
ENZYMES
ESTERS
FISHES
KINETICS
LABELLED COMPOUNDS
LIPIDS
MAMMALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
OXIDOREDUCTASES
REACTION KINETICS
RODENTS
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques