Autoradiography of phosphatidyl choline

Journal Article · Sun Mar 01 00:00:00 EST 1981 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/29.3.7240719· OSTI ID:5527581

Saffitz, J E; Gross, R W; Williamson, J R; Sobel, B E

Saturated choline phosphatides are extracted during conventional tissue processing for electron microscopy. To facilitate autoradiographic subcellular localization of arrhythmogenic myocardial phospholipids, we evaluated tissue processing procedures for preservation of saturated phosphatidyl choline (PC). Suspensions, of a murine plasmacytoma were incubated with negative, unilamellar liposomes containing 14C-choline-labeled PC or 14C-1-palmitate dipalmitoyl PC. Extraction of radioactivity was monitored at each processing step by liquid scintillation spectrometry. Conventional fixation with glutaraldehyde and osmium tetroxide followed by acetone dehydration and Spurr's plastic embedding led to extraction of nearly all radioactivity. However, treatment of cells with 1.5% tannic acid after glutaraldehyde but before osmium tetroxide fixation preserved 93.1 +/- .6% of 14C-choline-labeled PC. Virtually identical results were obtained with dipalmitoyl PC. Autoradiography demonstrated no significant translocation of labeled PC from plasmacytoma cells to unlabeled avian erythrocytes, mixed in equal proportions after fixation but before dehydration and embedding.

• OSTI ID: 5527581
• Journal Information: J. Histochem. Cytochem.; (United States), Vol. 29:3
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBON 14 COMPOUNDS
ISOTOPE APPLICATIONS
PHOSPHOLIPIDS
AUTORADIOGRAPHY
BIOLOGICAL LOCALIZATION
CHOLINE
MYOCARDIUM
SAMPLE PREPARATION
ALCOHOLS
AMINES
AMMONIUM COMPOUNDS
BODY
CARDIOVASCULAR SYSTEM
DRUGS
ESTERS
HEART
HYDROXY COMPOUNDS
LABELLED COMPOUNDS
LIPIDS
LIPOTROPIC FACTORS
MUSCLES
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
QUATERNARY COMPOUNDS
550201* - Biochemistry- Tracer Techniques