Manganese toxicity to chlorophyll synthesis in tobacco callus. [Nicotiana tabacum]

Journal Article · Wed Jan 01 00:00:00 EST 1986 · Plant Physiol.; (United States)

OSTI ID:5527460

Clairmont, K B; Hagar, W G; Davis, E A

Tobacco (Nicotiana tabacum) pith explants were grown on manganese containing medium. At moderate concentration (10 millimolar), manganese selectivity inhibited chlorophyll synthesis, resulting initially in growth of white callus. Several weeks later the white callus turned brown due to the accumulation of a pigment identified as protoporphyrin IX by its elution profile using high performance liquid chromatography, by its absorption spectrum, and by its fluorescence properties. At a concentration of 100 millimolar manganese the pigment accumulated without growth of the explant.

• Research Organization: Univ. of Massachusetts, Boston
• OSTI ID: 5527460
• Journal Information: Plant Physiol.; (United States), Vol. 80:1
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
CHLOROPHYLL
PHOTOSYNTHESIS
MANGANESE
TOXICITY
INHIBITION
ABSORPTION SPECTRA
BIOLOGICAL ACCUMULATION
EXPERIMENTAL DATA
FLUORESCENCE
LIQUID COLUMN CHROMATOGRAPHY
NICOTIANA
PLANT GROWTH
PRECURSOR
PROTOPORPHYRINS
CARBOXYLIC ACIDS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DATA
ELEMENTS
GROWTH
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INFORMATION
LUMINESCENCE
METALS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHYTOCHROMES
PIGMENTS
PLANTS
PORPHYRINS
PROTEINS
SEPARATION PROCESSES
SPECTRA
SYNTHESIS
TRANSITION ELEMENTS
560303* - Chemicals Metabolism & Toxicology- Plants- (-1987)
550500 - Metabolism