Radioimmunoassay for herpes simplex virus (HSV) thymidine kinase
A sensitive RIA for HSV-1 thymidine kinase (TK) has been developed. This assay is based on competition for the binding site of a rabbit antibody against purified HSV-1 TK, between a purified /sup 3/H-labeled HSV-1 TK and a sample containing an unknown amount of viral TK. The assay is capable of detecting 8 ng or more of the HSV enzyme. Purified HSV-1 TK denatured to <1% of its original kinase activity is as effective in binding to the antibody as is native HSV-1 TK. Viral TK is detectable at ranges of 150-460 ng/mg protein of cell extract from infected cells or cells transformed by HSV or HSV genetic material. HSV-2 TK appears highly cross-reactive, VZV TK is slightly less so, and the vaccinia TK shows little or no cross-reactivity. This RIA may serve as a tool for monitoring the expression of the HSV TK during an active herpes virus infection, a latent ganglionic infection, or in neoplastic cells which may have arisen by viral transformation.
- Research Organization:
- Burroughs Wellcome Co., Research Triangle Park, NC
- OSTI ID:
- 5527294
- Journal Information:
- Virology; (United States), Vol. 116:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HERPES SIMPLEX
PHOSPHOTRANSFERASES
RADIOIMMUNOASSAY
ANTIBODIES
BIOCHEMICAL REACTION KINETICS
COMPETITION
ENZYME ACTIVITY
VIRUSES
DISEASES
ENZYMES
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
KINETICS
MICROORGANISMS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
RADIOASSAY
REACTION KINETICS
SKIN DISEASES
TRACER TECHNIQUES
TRANSFERASES
VIRAL DISEASES
550601* - Medicine- Unsealed Radionuclides in Diagnostics