Oxidant-mediated ciliary dysfunction. Possible role in airway disease
The effects of reactive species of oxygen on the airway are not well known. This study examined the effects of hydrogen peroxide (H2O2) on the structure and function of the airway epithelium. Tracheal rings were prepared from 200 g male rats. Damage to the airway epithelium was assayed by monitoring the ciliary beat frequency, the release of 51Cr, and histology. H2O2 at concentrations of 1.0 mM and above caused a very rapid decrease in ciliary beat frequency. After ten minutes' exposure to 1.0 mM, the ciliary beat frequency was 72 +/- 20 percent of control. Release of 51Cr was a less sensitive measure with significant release occurring after four hours of exposure to ciliotoxic concentrations of H2O2. Histologic changes were not evident within the experimental time period. All toxic effects of H2O2 were completely blocked by catalase. This study shows that H2O2 causes a rapid decline in ciliary activity and suggests that oxidant-mediated ciliary dysfunction could play a role in the pathogenesis of airway disease. The ciliary beat frequency provides a sensitive, physiologically relevant parameter for the in vitro study of these diseases.
- Research Organization:
- Dept. of Internal Medicine Mayo Clinic, Rochester, MN
- OSTI ID:
- 5527237
- Journal Information:
- Chest; (United States), Vol. 3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
HYDROGEN PEROXIDE
TOXICITY
RESPIRATORY SYSTEM DISEASES
ETIOLOGY
TRACHEA
PATHOLOGICAL CHANGES
CHROMIUM 51
EPITHELIUM
LARYNX
RATS
TRACER TECHNIQUES
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BODY
CHROMIUM ISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
NUCLEI
OXYGEN COMPOUNDS
PEROXIDES
RADIOISOTOPES
RESPIRATORY SYSTEM
RODENTS
TISSUES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)