Absorption of D-[{sup 14}C]-glucose and D-[{sup 3}H]-mannose in everted rat jejunum

Wagner, B; Galey, Jr, W R

Title: Absorption of D-[{sup 14}C]-glucose and D-[{sup 3}H]-mannose in everted rat jejunum

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Abstract

Radiolabeled elements are useful in measuring physiologic processes in mammalian tissues. Absorption of D-[{sup 14}C]-glucose is often used as a marker for active transport by the sodium-dependent transmembrane glucose/galactose carrier, SGLT1, present in epithelial tissues. Also, D-[{sup 3}H]-mannose monitors the flux of a passively absorbed hexose through the intestinal wall. Many gastrointestinal absorption studies have employed the everted gut sac method developed by Wilson and Wiseman in 1954. Phloridzin is a glycoside derived from apple trees and an effective inhibitor of the sodium-dependent glucose carrier. It was hypothesized that a kinetic study of radiolabeled hexoses could be successfully applied to a modification of this method and assayed by an inhibitory effect of phloridzin on D-[{sup 14}C]-glucose influx into everted rat jejunum and that glycosides transported by the sodium-dependent glucose transporter would have a similar inhibitory effect on D-[{sup 14}C]-glucose influx.

Authors:

Wagner, B; Galey, Jr, W R ^[1]

Univ. of New Mexico, Albuquerque, NM (United States)

Publication Date:: Mon Dec 01 00:00:00 EST 1997

OSTI Identifier:: 552706

Report Number(s):: CONF-971125-
Journal ID: TANSAO; ISSN 0003-018X; TRN: 98:001911

Resource Type:: Journal Article

Journal Name:: Transactions of the American Nuclear Society

Additional Journal Information:: Journal Volume: 77; Conference: 1997 American Nuclear Society (ANS) winter meeting, Albuquerque, NM (United States), 16-20 Nov 1997; Other Information: PBD: 1997

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; GLUCOSE; ABSORPTION; MANNOSE; TRACER TECHNIQUES; LABELLED COMPOUNDS; CARBON 14; GALACTOSE; GLYCOSIDES; RATS; MEMBRANE TRANSPORT

Citation Formats


                    Wagner, B, and Galey, Jr, W R. Absorption of D-[{sup 14}C]-glucose and D-[{sup 3}H]-mannose in everted rat jejunum.  United States: N. p., 1997. 
        Web.

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                    Wagner, B, & Galey, Jr, W R. Absorption of D-[{sup 14}C]-glucose and D-[{sup 3}H]-mannose in everted rat jejunum.  United States.

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                    Wagner, B, and Galey, Jr, W R. 1997.  
        "Absorption of D-[{sup 14}C]-glucose and D-[{sup 3}H]-mannose in everted rat jejunum".  United States.

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@article{osti_552706,

  title        = {Absorption of D-[{sup 14}C]-glucose and D-[{sup 3}H]-mannose in everted rat jejunum},

  author       = {Wagner, B and Galey, Jr, W R},

  abstractNote = {Radiolabeled elements are useful in measuring physiologic processes in mammalian tissues. Absorption of D-[{sup 14}C]-glucose is often used as a marker for active transport by the sodium-dependent transmembrane glucose/galactose carrier, SGLT1, present in epithelial tissues. Also, D-[{sup 3}H]-mannose monitors the flux of a passively absorbed hexose through the intestinal wall. Many gastrointestinal absorption studies have employed the everted gut sac method developed by Wilson and Wiseman in 1954. Phloridzin is a glycoside derived from apple trees and an effective inhibitor of the sodium-dependent glucose carrier. It was hypothesized that a kinetic study of radiolabeled hexoses could be successfully applied to a modification of this method and assayed by an inhibitory effect of phloridzin on D-[{sup 14}C]-glucose influx into everted rat jejunum and that glycosides transported by the sodium-dependent glucose transporter would have a similar inhibitory effect on D-[{sup 14}C]-glucose influx.},

  doi          = {},

  url          = {https://www.osti.gov/biblio/552706},
  journal      = {Transactions of the American Nuclear Society},
number       = ,

  volume       = 77,

  place        = {United States},

  year         = {Mon Dec 01 00:00:00 EST 1997},

  month        = {Mon Dec 01 00:00:00 EST 1997}

}

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