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Title: Biochemical studies of DNA strand break repair and molecular characterization of mei-41, a gene involved in DNA break repair

Abstract

The ability to repair X-irradiation induced single-strand DNA breaks was examined in mutagen-sensitive mutants of Drosophila melanogaster. This analysis demonstrated that examined stocks possess a normal capacity to repair X-ray induced single-strand breaks. One of the mutants in this study, mei-41, has been shown to be involved in a number of DNA metabolizing functions. A molecular characterization of this mutant is presented. A cDNA hybridizing to genomic DNA both proximal and distal to a P element inducing a mei-41 mutation was isolated from both embryonic and adult female recombinant lambda phage libraries. A 2.2 kilobase embryonic cDNA clone was sequenced; the sequence of an open reading frame was identified which would predict a protein of 384 amino acids with a molecular weight of 43,132 daltons. An examination of homologies to sequences in protein and nucleic acid data bases revealed no sequences with significant homology to mei-41, however, two potential Zinc-finger domains were identified. Analysis of RNA hybridizing to the embryonic cDNA demonstrated the existence of a major 2.2 kilobase transcript expressed primarily in embryos and adult flies. An examination of the transcription of this gene in mei-41 mutants revealed significant variation from wild-type, an indication that the embryonic cDNA doesmore » represent a mei-41 transcript. Expression in tissues from adult animals demonstrated that the 2.2 kilobase RNA is expressed primarily in reproductive tissues. A 3.8kb transcript is the major species of RNA in the adult head and thorax. Evidence is presented which implies that expression of the mei-41 gene is strongly induced by exposure of certain cells to mutagens.« less

Authors:
Publication Date:
Research Org.:
California Univ., Davis, CA (United States)
OSTI Identifier:
5524806
Resource Type:
Miscellaneous
Resource Relation:
Other Information: Thesis (Ph. D.)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; DNA REPAIR; GENES; DROSOPHILA; STRAND BREAKS; RADIOINDUCTION; AMINO ACID SEQUENCE; DNA HYBRIDIZATION; GENE MUTATIONS; MOLECULAR BIOLOGY; RNA; X RADIATION; ANIMALS; ARTHROPODS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; DIPTERA; ELECTROMAGNETIC RADIATION; FLIES; FRUIT FLIES; HYBRIDIZATION; INSECTS; INVERTEBRATES; IONIZING RADIATIONS; MOLECULAR STRUCTURE; MUTATIONS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; RADIATIONS; RECOVERY; REPAIR; 560152* - Radiation Effects on Animals- Animals

Citation Formats

Oliveri, D R. Biochemical studies of DNA strand break repair and molecular characterization of mei-41, a gene involved in DNA break repair. United States: N. p., 1989. Web.
Oliveri, D R. Biochemical studies of DNA strand break repair and molecular characterization of mei-41, a gene involved in DNA break repair. United States.
Oliveri, D R. 1989. "Biochemical studies of DNA strand break repair and molecular characterization of mei-41, a gene involved in DNA break repair". United States.
@article{osti_5524806,
title = {Biochemical studies of DNA strand break repair and molecular characterization of mei-41, a gene involved in DNA break repair},
author = {Oliveri, D R},
abstractNote = {The ability to repair X-irradiation induced single-strand DNA breaks was examined in mutagen-sensitive mutants of Drosophila melanogaster. This analysis demonstrated that examined stocks possess a normal capacity to repair X-ray induced single-strand breaks. One of the mutants in this study, mei-41, has been shown to be involved in a number of DNA metabolizing functions. A molecular characterization of this mutant is presented. A cDNA hybridizing to genomic DNA both proximal and distal to a P element inducing a mei-41 mutation was isolated from both embryonic and adult female recombinant lambda phage libraries. A 2.2 kilobase embryonic cDNA clone was sequenced; the sequence of an open reading frame was identified which would predict a protein of 384 amino acids with a molecular weight of 43,132 daltons. An examination of homologies to sequences in protein and nucleic acid data bases revealed no sequences with significant homology to mei-41, however, two potential Zinc-finger domains were identified. Analysis of RNA hybridizing to the embryonic cDNA demonstrated the existence of a major 2.2 kilobase transcript expressed primarily in embryos and adult flies. An examination of the transcription of this gene in mei-41 mutants revealed significant variation from wild-type, an indication that the embryonic cDNA does represent a mei-41 transcript. Expression in tissues from adult animals demonstrated that the 2.2 kilobase RNA is expressed primarily in reproductive tissues. A 3.8kb transcript is the major species of RNA in the adult head and thorax. Evidence is presented which implies that expression of the mei-41 gene is strongly induced by exposure of certain cells to mutagens.},
doi = {},
url = {https://www.osti.gov/biblio/5524806}, journal = {},
number = ,
volume = ,
place = {United States},
year = {Sun Jan 01 00:00:00 EST 1989},
month = {Sun Jan 01 00:00:00 EST 1989}
}

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