Neuropeptide Y administration acutely increases hypothalamic corticotropin-releasing factor immunoreactivity: lack of effect in other rat brain regions

Journal Article · Mon Dec 21 00:00:00 EST 1987 · Life Sci.; (United States)

DOI:https://doi.org/10.1016/0024-3205(87)90465-6· OSTI ID:5520965

Haas, D A; George, S R

The effect of acute central administration of Neuropeptide Y (NPY) to adult male rats on the brain content of corticotropin-releasing factor immunoreactivity (CRF-ir) was investigated. The brain regions studied included frontal cortex, hippocampus, medulla-pons, midbrain-thalamus, cerebellum, neurointermediate lobe of pituitary, median eminence and the remaining hypothalamus. CRF-ir was determined in each of these regions using radioimmunoassay specific for rat CRF. CRF-ir was found to be significantly increased in the major site of CRF localization in the brain, the hypothalamus, in NPY-treated rats as compared to vehicle-treated controls either 15 minutes (p<0.025) or 45 minutes (p<0.005) post-injection. This increase was localized to the median eminence (p<0.05 after 15 minutes, p<0.01 after 45 minutes). No statistically significant differences were noted in any of the other brain regions assessed. Plasma adrenocorticotropin levels were also found to increase following NPY treatment, an effect which became significant after 45 minutes (p<0.05). These data show that NPY can alter the content of hypothalamic CRF and may play a role in its regulation. 33 references, 4 figures.

• Research Organization: Univ. of Toronto, Ontario
• OSTI ID: 5520965
• Journal Information: Life Sci.; (United States), Vol. 41:25
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PEPTIDES
BIOLOGICAL FUNCTIONS
BIOSYNTHESIS
RADIOIMMUNOASSAY
BRAIN
EXPERIMENTAL DATA
HYPOTHALAMUS
IMMUNE REACTIONS
RATS
ANIMALS
BODY
CENTRAL NERVOUS SYSTEM
DATA
FUNCTIONS
IMMUNOASSAY
IMMUNOLOGY
INFORMATION
ISOTOPE APPLICATIONS
MAMMALS
NERVOUS SYSTEM
NUMERICAL DATA
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
RODENTS
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques