Changes in inositol phosphates in wild carrot cells upon initiation of cell wall digestion
Previous studies have shown that inositol trisphosphate (IP/sub 3/) stimulated /sup 45/Ca/sup +2/ efflux from fusogenic carrot protoplasts and it was suggested that IP/sub 3/ may serve as a second messenger for the mobilization of intracellular Ca/sup +2/ in higher plant cells. To determine whether or not inositol phosphate metabolism changes in response to external stimuli, the cells were labeled with myo-(2-/sup 3/H) inositol for 18 h and exposed to cell wall digestion enzymes, Driselase. The inositol phosphates were extracted with ice cold 10% TCA and separated by anion exchange chromatography. The radioactivity of the fraction that contained IP/sub 3/ increased 2-3.8 fold and that which contained inositol bisphosphate increased 1.9-2.6 fold within 1.5 min of exposure to Driselase. After 6 min, the radioactivity of both fractions increased 6-7.7 fold and an increase in inositol monophosphate was observed. These data indicate that inositol phosphate metabolism is stimulated by Driselase and suggest polyphosphoinositide hydrolysis occurs upon initiation of cell wall digestion.
- Research Organization:
- North Carolina State Univ., Raleigh
- OSTI ID:
- 5520561
- Report Number(s):
- CONF-8707108-
- Journal Information:
- Plant Physiol., Suppl.; (United States), Vol. 83:4; Conference: Annual meeting of the American Society of Plant Physiologists, St. Louis, MO, USA, 19 Jul 1987
- Country of Publication:
- United States
- Language:
- English
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IP/sub 3/ stimulates CA/sup + +/ efflux from fusogenic carrot protoplasts
Myo-Inositol trisphosphate mobilizes calcium from fusogenic carrot (Daucus carota L. ) protoplasts
Related Subjects
INOSITOL
METABOLISM
ORGANIC PHOSPHORUS COMPOUNDS
CALCIUM
CARROTS
CATIONS
CELL WALL
DIGESTION
ION EXCHANGE CHROMATOGRAPHY
PLANT CELLS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALINE EARTH METALS
CARBOHYDRATES
CELL CONSTITUENTS
CHARGED PARTICLES
CHROMATOGRAPHY
ELEMENTS
FOOD
INOSITOLS
IONS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
METALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
SACCHARIDES
SEPARATION PROCESSES
VEGETABLES
550201* - Biochemistry- Tracer Techniques