Comparison of altered expression of histocompatibility antigens with altered immune function in murine spleen cells treated with ultraviolet radiation and/or TPA
Previous studies in our laboratory demonstrated that several treatments that inhibited the ability of cells to stimulate the mixed lymphocyte reaction (MLR) also blocked the shedding of histocompatibility antigens and Ia antigens from murine spleen cells. In the present studies, one of these treatments, ultraviolet radiation (UV), was shown to cause an initial loss in the density of H-2K, IA, and IE antigens prior to the block in shedding observed after culture of these cells. Further analysis revealed that the UV-induced loss of antigens could be prevented by the presence of colchicine during irradiation. Biosynthetic analyses revealed the IA antigen synthesis was also inhibited in the UV-irradiated cells. Examination of the effects of a second agent, 12-0-tetradecanoylphorbol-13-acetate (TPA) on the turnover of histocompatibility antigens revealed that the biosynthesis and shedding of these antigens were accelerated by this agent. However, addition of TPA to UV-irradiated cells did not result in a reversal of the UV-induced block in biosynthesis of IA antigens. Results of immune function assays correlated with the biochemical studies: UV-irradiation inhibited the generation of the MLR, but TPA enhanced this reaction, and addition of TPA to mixed lymphocyte cultures with UV-irradiated stimulators did not reverse the UV-induced inhibition. These results suggest that, although the turnover of histocompatibility antigens may be affected by TPA and UV in an antagonistic fashion, additional factors other than the expression of histocompatibility antigens are operating in the inhibition of stimulation of an MLR by UV radiation or its enhancement by TPA.
- Research Organization:
- Yale Univ. School of Medicine, New Haven, CT
- OSTI ID:
- 5520499
- Journal Information:
- Transplantation; (United States), Vol. 39:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ACETATES
BIOLOGICAL EFFECTS
ANTIGENS
BIOSYNTHESIS
COLCHICINE
RADIOSENSITIVITY EFFECTS
LYMPHOCYTES
BIOLOGICAL RADIATION EFFECTS
SPLEEN CELLS
CELL CULTURES
COMPARATIVE EVALUATIONS
COMPATIBILITY
IMMUNE REACTIONS
PHORBOL ESTERS
ULTRAVIOLET RADIATION
ALKALOIDS
ANIMAL CELLS
ANTIMITOTIC DRUGS
ANTIPYRETICS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACID SALTS
CARCINOGENS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
CONNECTIVE TISSUE CELLS
DRUGS
ELECTROMAGNETIC RADIATION
ESTERS
LEUKOCYTES
MATERIALS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS
SYNTHESIS
560121* - Radiation Effects on Cells- External Source- (-1987)
560301 - Chemicals Metabolism & Toxicology- Cells- (-1987)