Tissue-specific expression and cDNA cloning of small nuclear ribonucleoprotein-associated polypeptide N
Abstract
Sera from some patients with systemic lupus erythematosus and other autoimmune diseases have antibodies against nuclear antigens. An example is anti-Sm sera, which recognize proteins associated with small nuclear RNA molecules (small nuclear ribonucleoprotein (snRNP) particles). In this paper anti-Sm sera were used to probe immunoblots of various rat tissues. A previously unidentified M{sub r} 28,000 polypeptide was recognized by these anti-Sm sera. This polypeptide, referred to as N, is expressed in a tissue-specific manner, being most abundant in rat brain, less so in heart, and undetectable in the other tissues examined. Immunoprecipitation experiments using antibodies directed against the cap structure of small nuclear RNAs have demonstrated that N is a snRNP-associated polypeptide. Anti-Sm serum was also used to isolate a partial cDNA clone ({lambda}rb91) from a rat brain phage {lambda}gt11 cDNA expression library. A longer cDNA clone was obtained by rescreening the library with {lambda}rb91. In vitro transcription and subsequent translation of this subcloned, longer insert (pGMA2) resulted in a protein product with the same electrophoretic and immunological properties as N, confirming that pGMA2 encodes N. The tissue distribution of N and the involvement of snRNP particles in nuclear pre-mRNA processing may imply a role for N in tissue-specificmore »
- Authors:
-
- Yale Univ. School of Medicine, New Haven, CT (USA)
- Publication Date:
- OSTI Identifier:
- 5517616
- Resource Type:
- Journal Article
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America; (USA)
- Additional Journal Information:
- Journal Volume: 85:14; Journal ID: ISSN 0027-8424
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; NUCLEOPROTEINS; TISSUE DISTRIBUTION; RECOMBINANT DNA; DNA-CLONING; RHEUMATIC DISEASES; IMMUNOLOGY; ANTIBODIES; BRAIN; DNA SEQUENCING; ELECTROPHORESIS; HEART; PATIENTS; PHOSPHORUS 32; RATS; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CARDIOVASCULAR SYSTEM; CENTRAL NERVOUS SYSTEM; CLONING; DAYS LIVING RADIOISOTOPES; DISEASES; DISTRIBUTION; DNA; DNA HYBRIDIZATION; HYBRIDIZATION; ISOTOPES; LIGHT NUCLEI; MAMMALS; NERVOUS SYSTEM; NUCLEI; NUCLEIC ACIDS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; PROTEINS; RADIOISOTOPES; RODENTS; SKELETAL DISEASES; STRUCTURAL CHEMICAL ANALYSIS; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques
Citation Formats
McAllister, G, Amara, S G, and Lerner, M R. Tissue-specific expression and cDNA cloning of small nuclear ribonucleoprotein-associated polypeptide N. United States: N. p., 1988.
Web. doi:10.1073/pnas.85.14.5296.
McAllister, G, Amara, S G, & Lerner, M R. Tissue-specific expression and cDNA cloning of small nuclear ribonucleoprotein-associated polypeptide N. United States. https://doi.org/10.1073/pnas.85.14.5296
McAllister, G, Amara, S G, and Lerner, M R. 1988.
"Tissue-specific expression and cDNA cloning of small nuclear ribonucleoprotein-associated polypeptide N". United States. https://doi.org/10.1073/pnas.85.14.5296.
@article{osti_5517616,
title = {Tissue-specific expression and cDNA cloning of small nuclear ribonucleoprotein-associated polypeptide N},
author = {McAllister, G and Amara, S G and Lerner, M R},
abstractNote = {Sera from some patients with systemic lupus erythematosus and other autoimmune diseases have antibodies against nuclear antigens. An example is anti-Sm sera, which recognize proteins associated with small nuclear RNA molecules (small nuclear ribonucleoprotein (snRNP) particles). In this paper anti-Sm sera were used to probe immunoblots of various rat tissues. A previously unidentified M{sub r} 28,000 polypeptide was recognized by these anti-Sm sera. This polypeptide, referred to as N, is expressed in a tissue-specific manner, being most abundant in rat brain, less so in heart, and undetectable in the other tissues examined. Immunoprecipitation experiments using antibodies directed against the cap structure of small nuclear RNAs have demonstrated that N is a snRNP-associated polypeptide. Anti-Sm serum was also used to isolate a partial cDNA clone ({lambda}rb91) from a rat brain phage {lambda}gt11 cDNA expression library. A longer cDNA clone was obtained by rescreening the library with {lambda}rb91. In vitro transcription and subsequent translation of this subcloned, longer insert (pGMA2) resulted in a protein product with the same electrophoretic and immunological properties as N, confirming that pGMA2 encodes N. The tissue distribution of N and the involvement of snRNP particles in nuclear pre-mRNA processing may imply a role for N in tissue-specific pre-mRNA splicing.},
doi = {10.1073/pnas.85.14.5296},
url = {https://www.osti.gov/biblio/5517616},
journal = {Proceedings of the National Academy of Sciences of the United States of America; (USA)},
issn = {0027-8424},
number = ,
volume = 85:14,
place = {United States},
year = {Fri Jul 01 00:00:00 EDT 1988},
month = {Fri Jul 01 00:00:00 EDT 1988}
}