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Title: A milk protein gene promoter directs the expression of human tissue plasminogen activator cDNA to the mammary gland in transgenic mice

Abstract

Whey acidic protein (WAP) is a major whey protein in mouse milk. Its gene is expressed in the lactating mammary gland and is inducible by steroid and peptide hormones. A series of transgenic mice containing a hybrid gene in which human tissue plasminogen activator (tPA) cDNA is under the control of the murine WAP gene promoter had previously been generated. In this study, 21 tissues from lactating and virgin transgenic female mice containing the WAP-tPA hybrid gene were screened for the distribution of murine WAP and human tPA transcripts. Like the endogenous WAP RNA, WAP-tPA RNA was expressed predominantly in mammary gland tissue and appeared to be inducible by lactation. Whereas WAP transcripts were not detected in 22 tissues of virgin mice, low levels of WAP-tPA RNA, which were not modulated during lactation, were found in tongue, kidney, and sublingual gland. These studies demonstrate that the WAP gene promoter can target the expression of a transgene to the mammary gland and that this expression is inducible during lactation.

Authors:
; ; ; ; ; ;  [1]
  1. National Institutes of Health, Bethesda, MD (USA)
Publication Date:
OSTI Identifier:
5517476
Resource Type:
Journal Article
Journal Name:
Proceedings of the National Academy of Sciences of the United States of America; (USA)
Additional Journal Information:
Journal Volume: 85:16; Journal ID: ISSN 0027-8424
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; GENE REPRESSORS; TRANSCRIPTION; LACTATION; BIOCHEMISTRY; PEPTIDE HYDROLASES; GENE REGULATION; WHEY; CHEMICAL ACTIVATION; ELECTROPHORESIS; HYBRIDIZATION; MAMMARY GLANDS; MAN; MICE; PHOSPHORUS 32; PLASMINOGEN; RECOMBINANT DNA; ANIMALS; BETA DECAY RADIOISOTOPES; BETA-MINUS DECAY RADIOISOTOPES; BODY; CHEMISTRY; DAYS LIVING RADIOISOTOPES; DNA; DRUGS; ENZYMES; FIBRINOLYTIC AGENTS; FOOD; GLANDS; HEMATOLOGIC AGENTS; HYDROLASES; INDUSTRIAL WASTES; ISOTOPES; LIGHT NUCLEI; MAMMALS; MILK PRODUCTS; NUCLEI; NUCLEIC ACIDS; NUCLEOPROTEINS; ODD-ODD NUCLEI; ORGANIC COMPOUNDS; ORGANS; PHOSPHORUS ISOTOPES; PRIMATES; PROTEINS; RADIOISOTOPES; RODENTS; VERTEBRATES; WASTES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Pittius, C W, Hennighausen, L, Lee, E, Westphal, H, Nicols, E, Vitale, J, and Gordon, K. A milk protein gene promoter directs the expression of human tissue plasminogen activator cDNA to the mammary gland in transgenic mice. United States: N. p., 1988. Web. doi:10.1073/pnas.85.16.5874.
Pittius, C W, Hennighausen, L, Lee, E, Westphal, H, Nicols, E, Vitale, J, & Gordon, K. A milk protein gene promoter directs the expression of human tissue plasminogen activator cDNA to the mammary gland in transgenic mice. United States. https://doi.org/10.1073/pnas.85.16.5874
Pittius, C W, Hennighausen, L, Lee, E, Westphal, H, Nicols, E, Vitale, J, and Gordon, K. 1988. "A milk protein gene promoter directs the expression of human tissue plasminogen activator cDNA to the mammary gland in transgenic mice". United States. https://doi.org/10.1073/pnas.85.16.5874.
@article{osti_5517476,
title = {A milk protein gene promoter directs the expression of human tissue plasminogen activator cDNA to the mammary gland in transgenic mice},
author = {Pittius, C W and Hennighausen, L and Lee, E and Westphal, H and Nicols, E and Vitale, J and Gordon, K},
abstractNote = {Whey acidic protein (WAP) is a major whey protein in mouse milk. Its gene is expressed in the lactating mammary gland and is inducible by steroid and peptide hormones. A series of transgenic mice containing a hybrid gene in which human tissue plasminogen activator (tPA) cDNA is under the control of the murine WAP gene promoter had previously been generated. In this study, 21 tissues from lactating and virgin transgenic female mice containing the WAP-tPA hybrid gene were screened for the distribution of murine WAP and human tPA transcripts. Like the endogenous WAP RNA, WAP-tPA RNA was expressed predominantly in mammary gland tissue and appeared to be inducible by lactation. Whereas WAP transcripts were not detected in 22 tissues of virgin mice, low levels of WAP-tPA RNA, which were not modulated during lactation, were found in tongue, kidney, and sublingual gland. These studies demonstrate that the WAP gene promoter can target the expression of a transgene to the mammary gland and that this expression is inducible during lactation.},
doi = {10.1073/pnas.85.16.5874},
url = {https://www.osti.gov/biblio/5517476}, journal = {Proceedings of the National Academy of Sciences of the United States of America; (USA)},
issn = {0027-8424},
number = ,
volume = 85:16,
place = {United States},
year = {Mon Aug 01 00:00:00 EDT 1988},
month = {Mon Aug 01 00:00:00 EDT 1988}
}