Cultured human foreskin fibroblasts produce a factor that stimulates their growth with properties similar to basic fibroblast growth factor

Journal Article · Mon May 01 00:00:00 EDT 1989 · In Vitro Cellular and Developmental Biology; (USA)

DOI:https://doi.org/10.1007/BF02624624· OSTI ID:5517052

Story, M T ^[1]

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1. Medical College of Wisconsin, Milwaukee (USA)

To determine if fibroblasts could be a source of fibroblast growth factor (FGF) in tissue, cells were initiated in culture from newborn human foreskin. Fibroblast cell lysates promoted radiolabeled thymidine uptake by cultured quiescent fibroblasts. Seventy-nine percent of the growth-promoting activity of lysates was recovered from heparin-Sepharose. The heparin-binding growth factor reacted on immunoblots with antiserum to human placenta-derived basic FGF and competed with iodinated basic FGF for binding to antiserum to (1-24)bFGF synthetic peptide. To confirm that fibroblasts were the source of the growth factor, cell lysates were prepared from cells incubated with radiolabeled methionine. Heparin affinity purified material was immunoprecipitated with basic FGF antiserum and electrophoresed. Radiolabeled material was detected on gel autoradiographs in the same molecular weight region as authentic iodinated basic FGF. The findings are consistant with the notion that cultured fibroblasts express basic FGF. As these cells also respond to the mitogen, it is possible that the regulation of their growth is under autocrine control. Fibroblasts may be an important source of the growth factor in tissue.

• OSTI ID: 5517052
• Journal Information: In Vitro Cellular and Developmental Biology; (USA), Vol. 25:5; ISSN 0883-8364
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
FIBROBLASTS
CELL DIVISION
GROWTH FACTORS
BIOCHEMICAL REACTION KINETICS
AUTORADIOGRAPHY
CELL CULTURES
ELECTROPHORESIS
IMMUNOASSAY
INFANTS
MAN
METHIONINE
SULFUR ISOTOPES
THYMIDINE
AGE GROUPS
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AZINES
BIOASSAY
CARBOXYLIC ACIDS
CHILDREN
CONNECTIVE TISSUE CELLS
DRUGS
HETEROCYCLIC COMPOUNDS
ISOTOPES
KINETICS
LIPOTROPIC FACTORS
MAMMALS
MITOGENS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PRIMATES
PROTEINS
PYRIMIDINES
REACTION KINETICS
RIBOSIDES
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques