Microbial penetration through Berea sandstone and the effect of nitrate on biogenic sulfide production: their relevance to microbial enhanced oil recovery

Thesis/Dissertation · Tue Jan 01 00:00:00 EST 1985

OSTI ID:5516509

Jenneman, G E

Penetration times, and penetration rates, for a motile Bacillus strain growing in nutrient-saturated Berea sandstone cores were determined. The rate of penetration was essentially independent of permeability above 100 mD and rapidly declined for permeabilities below 100 mD. The maximum observed penetration rate was 0.47 cm/h and the slowest was 0.06 xm/h. The motile Enterobacter aerrogenes penetrated Berea sandstone cores 3-8 times faster than the non-motile Klebsiella pneumoniae strain when cores of comparable length of permeability were used. Results from energy dispersion spectroscopy, and electron microscopy of autoclaved and dry-heat sterilized rock revealed that changes in mineral content and clay morphology of autoclaved rock could account for faster penetration of bacteria through autoclaved versus dry-heat sterilized rock. However, changes in permeability, porosity, and pore throat size of rock sterilized by the two methods were not sufficient to account for differences in penetration times. The addition of 58.8 mM nitrate was found to inhibit biogenic sulfide production in sewage sludge (10% v/v) amended with 20 mM sulfate and either acetate, glucose, or hydrogen as energy sources. Lesser amounts of nitrate (5.9 mM and 19.5 mM), as well as, increasing amounts of sewage sludge resulted in only transient inhibition of sulfide production. The addition of 156 mM sulfate and 58.8 mM nitrate to 10% sewage sludge or pond sediment slurries resulted in no sulfide inhibition. The prolonged inhibition of sulfide production was attributed to an increase in oxidation-reduction potential due to production of biogenic nitrous oxide. These oxidized conditions appeared to have a cytotoxic affect on sulfate-reducing populations.

• Research Organization: Oklahoma Univ., Norman (USA)
• OSTI ID: 5516509
• Resource Relation: Other Information: Thesis (Ph. D.)
• Country of Publication: United States
• Language: English

Related Subjects

02 PETROLEUM
59 BASIC BIOLOGICAL SCIENCES
MICROBIAL EOR
SULFATE-REDUCING BACTERIA
INHIBITION
PERFORMANCE TESTING
BACILLUS
BIOSYNTHESIS
DRILL CORES
KLEBSIELLA
NITRATES
PERMEABILITY
PETROLEUM
SANDSTONES
SEWAGE SLUDGE
SULFIDES
BACTERIA
CHALCOGENIDES
ENERGY SOURCES
ENHANCED RECOVERY
FOSSIL FUELS
FUELS
MICROORGANISMS
NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
RECOVERY
ROCKS
SEDIMENTARY ROCKS
SEWAGE
SLUDGES
SULFUR COMPOUNDS
SYNTHESIS
TESTING
WASTES
020300* - Petroleum- Drilling & Production
550700 - Microbiology