Radiolabeling antibodies and other proteins with technetium or rhenium by regulated reduction
This patent describes a method of radiolabeling a protein containing disulfide bonds with a radionuclide selected from the group consisting of technetium and rhenium to obtain stable labeling. It comprises incubating the protein containing disulfide bonds with a first reducing agent, the period of incubation being sufficient to reduce available disulfide bonds to sulfhydryl groups while preventing excessive fragmentation of the protein; purifying the reduced protein to substantially remove the first reducing agent and impurities; adding a source of stannous ion agent to the reduced protein in a sufficient amount to reduce the radionuclide, the radionuclide to be added in a subsequent step; radiolabeling the purified reduced protein by adding the radionuclide, whereby the stannous ion agent reduces the radionuclide and the reduced radionuclide and reduced protein forms radionuclide-containing and sulfur-containing complexes.
- Assignee:
- RhoMed, Inc., Albuquerque, NM (United States)
- Patent Number(s):
- US 5079985; A
- Application Number:
- PPN: US 7-391474
- OSTI ID:
- 5515807
- Resource Relation:
- Patent File Date: 9 Aug 1989
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ANTIBODIES
REDUCTION
PROTEINS
RADIOISOTOPES
CHEMICAL BONDS
OPTIMIZATION
PURIFICATION
RHENIUM
STANNATES
SULFIDES
SULFUR COMPLEXES
TECHNETIUM
CHALCOGENIDES
CHEMICAL REACTIONS
COMPLEXES
ELEMENTS
ISOTOPES
METALS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
SULFUR COMPOUNDS
TIN COMPOUNDS
TRANSITION ELEMENTS
550200* - Biochemistry