N,N prime -Dimethylthiourea dioxide formation from N,N prime -dimethylthiourea reflects hydrogen peroxide concentrations in simple biological systems

Journal Article · Sun May 01 00:00:00 EDT 1988 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.85.10.3422· OSTI ID:5514029

Curtis, W E; Muldrow, M E; Parker, N B; Barkley, R; Linas, S L; Repine, J E ^[1]

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1. Univ. of Colorado Health Sciences Center, Denver (USA)

The authors hypothesized that measurement of a specific product from reaction of N,N{prime}-dimethylthiourea (Me{sub 2}TU) and H{sub 2}O{sub 2} would provide a good indication of the H{sub 2}O{sub 2} scavenging and protection seen after addition of Me{sub 2}TU to biological systems. They found that addition of H{sub 2}O{sub 2} to Me{sub 2}TU yielded a single stable product, Me{sub 2}TU dioxide. Me{sub 2}TU dioxide formation correlated with Me{sub 2}TU consumption as a function of added H{sub 2}O{sub 2} concentration and was prevented by simultaneous addition of catalase (but not boiled catalase), superoxide dismutase, dimethyl sulfoxide, mannitol, or sodium benzoate. Me{sub 2}TU dioxide formation, Me{sub 2}TU consumption, and H{sub 2}O{sub 2} concentration increases occurred in mixtures containing phorbol 12-myristate 13-acetate (PMA) and normal human neutrophils but not in mixtures containing PMA and neutrophils from patients with chronic granulomatous disease or in mixtures containing PMA and normal neutrophils and catalase. Me{sub 2}TU dioxide formation also occurred in isolated rat lungs perfused with Me{sub 2}TU and H{sub 2}O{sub 2} but not in lungs perfused with Me{sub 2}TU and elastase, histamine, or oleic acid. In contrast, Me{sub 2}TU dioxide formation did not occur after exposure of Me{sub 2}TU to {sup 60}Co-generated hydroxyl radical or hypochlorous acid in the presence of catalase. The results indicate that reaction of Me{sub 2}TU with H{sub 2}O{sub 2} selectively forms Me{sub 2}TU may be useful for assessing the presence and significance of H{sub 2}O{sub 2} in biological systems.

• OSTI ID: 5514029
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 85:10; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HYDROGEN PEROXIDE
BIOCHEMICAL REACTION KINETICS
LUNGS
BIOLOGICAL RADIATION EFFECTS
OXYGEN
METABOLITES
THIOUREA
CATALASE
COBALT 60
DMSO
GAMMA RADIATION
HYDROXYL RADICALS
LIQUID COLUMN CHROMATOGRAPHY
MAN
NEUTROPHILS
PERFUSED TISSUES
PHORBOL ESTERS
SUPEROXIDE DISMUTASE
ANIMAL TISSUES
ANIMALS
ANTITHYROID DRUGS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARBONIC ACID DERIVATIVES
CARCINOGENS
CHROMATOGRAPHY
COBALT ISOTOPES
DRUGS
ELECTROMAGNETIC RADIATION
ELEMENTS
ENZYMES
ESTERS
HORMONE ANTAGONISTS
HYDROGEN COMPOUNDS
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
KINETICS
LEUKOCYTES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NONMETALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGEN COMPOUNDS
PEROXIDASES
PEROXIDES
PRIMATES
RADIATION EFFECTS
RADIATIONS
RADICALS
RADIOISOTOPES
REACTION KINETICS
RESPIRATORY SYSTEM
SEPARATION PROCESSES
SULFOXIDES
THIOUREAS
TISSUES
VERTEBRATES
YEARS LIVING RADIOISOT
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture