Resonance Raman studies of the HOOP modes in octopus bathorhodopsin with deuterium-labeled retinal chromophores

Journal Article · Tue May 07 00:00:00 EDT 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00232a018· OSTI ID:5510664

Deng, H; Manor, D; Weng, G; Rath, P; Callender, R H ^[1]; Koutalos, Y; Ebrey, T ^[2]; Gebhard, R; Lugtenburg, J ^[3]; Tsuda, M ^[4]

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1. City College, New York, NY (USA)
2. Univ. of Illinois, Urbana (USA)
3. Univ. of Leiden (Netherlands)
4. Himeji Institute of Technology, Hyogo (Japan)

Resonance Raman spectra of the hydrogen out-of-plane (HOOP) vibrational modes in the retinal chromophore of octopus bathorhodopsin with deuterium label(s) along the polyene chain have been obtained. In clear contrast with bovine bathorhodopsin's HOOP modes, there are only two major HOOP bands at 887 and 940 cm{sup {minus}1} for octopus bathorhodopsin. On the basis of their isotopic shifts upon deuterium labeling, the authors have assigned the band at 887 cm{sup {minus}1} to C{sub 10}H and C{sub 14}H HOOP modes, and the band at 940 cm{sup {minus}1} to C{sub 11}H{double bond}C{sub 12}H A{sub u}-like HOOP mode. They found also that the C{sub 10}H and C{sub 14}H HOOP wags are also similar to those in the model-compound studies. However, they have found that the interaction between the C{sub 7}H and C{sub 8}H HOOP internal coordinates of the chromophore in octopus bathorhodopsin is different from that of the chromophore in solution. The twisted nature of the chromophore, semiquantitatively discussed here, likely affects the {lambda}{sub max} of the chromophore and its enthalpy. The nature of the HOOP modes of octopus bathorhodopsin differs substantially from those found in bovine bathorhodopsin.

• OSTI ID: 5510664
• Journal Information: Biochemistry; (United States), Vol. 30:18; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PIGMENTS
MOLECULAR STRUCTURE
RHODOPSIN
RAMAN SPECTROSCOPY
DEUTERIUM COMPOUNDS
INVERTEBRATES
RETINA
VISION
ANIMALS
BODY
BODY AREAS
EYES
FACE
HEAD
HYDROGEN COMPOUNDS
LASER SPECTROSCOPY
ORGANIC COMPOUNDS
ORGANS
PROTEINS
SENSE ORGANS
SPECTROSCOPY
550201* - Biochemistry- Tracer Techniques