Suitability of different silver enhancement methods applied to 1 nm colloidal gold particles: an immunoelectron microscopic study

Journal Article · Fri Mar 01 00:00:00 EST 1991 · Journal of Electron Microscopy Technique; (United States)

DOI:https://doi.org/10.1002/jemt.1060170307· OSTI ID:5509856

Stierhof, Y D; Humbel, B M; Schwarz, H ^[1]

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1. Max-Planck-Institut fuer Biologie, Tuebingen (Federal Republic of Germany)

In order to exploit the recently introduced 1 nm gold colloids in routine electron microscopic labeling experiments, an efficient enhancement step for a better visualization of this small marker is a prerequisite. Efficiency and reproducibility of enhancement as well as growth homogeneity of gold particles were evaluated for three different silver intensifying solutions: silver lactate/hydroquinone/gum arabic, and the commercially available IntenSE M silver enhancement kit. The best results were obtained by using the silver lactate/hydroquinone/gum arabic mixture. The quality of enhancement of the IntenSE M kit was considerably increased by the addition of the protective colloid gum arabic.

• OSTI ID: 5509856
• Journal Information: Journal of Electron Microscopy Technique; (United States), Vol. 17:3; ISSN 0741-0581
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ELECTRON MICROSCOPY
TRACER TECHNIQUES
COLLOIDS
EFFICIENCY
ESCHERICHIA COLI
GOLD ISOTOPES
LACTATES
SILVER
BACTERIA
CARBOXYLIC ACID SALTS
DISPERSIONS
ELEMENTS
ISOTOPE APPLICATIONS
ISOTOPES
METALS
MICROORGANISMS
MICROSCOPY
TRANSITION ELEMENTS
550201* - Biochemistry- Tracer Techniques