Atrial natriuretic factor: radioimmunoassay and effects on adrenal and pituitary glands

Journal Article · Sun Jun 01 00:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5509459

Gutkowska, J; Horky, K; Schiffrin, E L; Thibault, G; Garcia, R; De Lean, A; Hamet, P; Tremblay, J; Anand-Srivastava, M B; Januszewicz, P

A simple and sensitive radioimmunoassay was developed for measurement of immunoreactive atrial natriuretic factor (IR-ANF) in rat and human plasma and in rat atria. The two atria contain about 20 ..mu..g ANF per rat. The right atrium contained 2.5 times more ANF than did the left. Ether anesthesia and morphine markedly increased IR-ANF in rat plasma. The concentration of IR-ANF in plasma of clinically normal human subjects was 65.3 +/- 2.5 pg/ml. Paroxysmal tachycardia and rapid atrial pacing significantly increased IR-ANF in human plasma. Two- to seven-fold higher concentrations were found in coronary sinus blood than in the peripheral circulation. In the plasma of rats and humans, circulating ANF is probably a small-molecular-weight peptide. ANF acts on the adrenal and the pituitary. ANF inhibits aldosterone secretion from rat zona glomerulosa and steroid secretion by bovine adrenal zona glomerulosa and fasciculata. ANF stimulated the basal secretion of arginine vasopressin (AVP) in vitro and inhibited KCl-stimulated release of AVP.

• Research Organization: Clinical Research Institute of Montreal, Quebec
• OSTI ID: 5509459
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:7
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ALDOSTERONE
SECRETION
PEPTIDES
BIOLOGICAL EFFECTS
RADIOIMMUNOASSAY
STEROIDS
VASOPRESSIN
ADRENAL GLANDS
BLOOD PLASMA
IMMUNE REACTIONS
INHIBITION
MAN
PITUITARY GLAND
RATS
ADRENAL HORMONES
ALDEHYDES
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BODY
BODY FLUIDS
CORTICOSTEROIDS
ENDOCRINE GLANDS
GLANDS
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
KETONES
MAMMALS
MATERIALS
MINERALOCORTICOIDS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PREGNANES
PRIMATES
PROTEINS
RADIOASSAY
RADIOIMMUNOLOGY
RODENTS
STEROID HORMONES
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques