Characterization of a human antigen specific helper factor
While antigen (Ag) specific helper factors have been characterized in mice, similar molecules have not been identified in humans. To characterize human antigen specific helper molecules, an IL-2 dependent tetanus toxoid (T.T.) reactive T cell line was fused with a 6-thioguanine resistant CEM line, and hybrids selected in medium containing hypoxanthine and azaserine. Hybrids were screened by culturing the cells with /sup 35/S-Met then reacting the supernatants with T.T. or hepatitis vaccine immobilized on nitrocellulose. One hybrid, TT6BA-O, was identified which secreted a Met-containing molecule which bound T.T. but not hepatitis vaccine. Supernatants from TT6BA-O, but not the parent CEM line, when added to autologous peripheral blood mononuclear cells (PBMC's) stimulated secretion of T.T. specific antibodies (Abs). Specificity controls demonstrated that TT6BA-O supernatant did not induce antibodies to diphtheria toxoid, hepatitis vaccine or pneumococcal polysaccharide, and total immunoglobulin (lg) synthesis was minimally increased. In contrast, pokeweed mitogen stimulated significant lg synthesis as well as Ab's to pneumococcal polysaccharide and T.T. TT6BA-O supernatant induced anti-T.T.Ab's in autologous PBMC's but not PBMC's from 3 unrelated donors, suggesting that the activity of the helper factor is restricted, possibly by the MHC. The molecular weight of the helper factor was estimated at 100,000-150,000 by Sephacryl S-300 chromatography. Finally, the helper factor could be demonstrated to bind and elute from sephorose-immobilized T.T. and anti-DR antisera, but not anti-lg antisera or the T40/25 monoclonal antibody, which binds a nonpolymorphic determinant on the human T cell receptor. These results demonstrate that human Ag specific helper factors exist, bind antigen and bear class II MHC determinants.
- Research Organization:
- Univ. of Michigan, Ann Arbor
- OSTI ID:
- 5508798
- Report Number(s):
- CONF-8604222-; TRN: 86-026571
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Specific suppression of anti-DNA production in vitro
Antibody production in rats after long-term exposure to formaldehyde
Related Subjects
BLOOD COAGULATION FACTORS
BIOCHEMICAL REACTION KINETICS
IMMUNOGLOBULINS
BIOSYNTHESIS
ANTIBODIES
ANTIGENS
CELL CULTURES
CHROMATOGRAPHY
HEPATITIS
LYMPHOCYTES
MOLECULAR WEIGHT
MOLECULES
MONOCLONAL ANTIBODIES
RECEPTORS
SULFUR 35
TRACER TECHNIQUES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
COAGULANTS
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM DISEASES
DISEASES
DRUGS
EVEN-ODD NUCLEI
GLOBULINS
HEMATOLOGIC AGENTS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
LIGHT NUCLEI
MATERIALS
MEMBRANE PROTEINS
NUCLEI
ORGANIC COMPOUNDS
PROTEINS
RADIOISOTOPES
REACTION KINETICS
SEPARATION PROCESSES
SOMATIC CELLS
SULFUR ISOTOPES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques