Calcium dependence of the induction of B cell class I molecules and of proliferation by various B cell mitogens

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5508555

Dennis, G; Mizuguchi, J; Finkelman, F D; Mond, J J

The concentration of calcium in the cytosol influences the metabolism of phosphoinositides which play essential roles as intracellular messengers in B lymphocytes. To investigate the calcium requirements for various stages of B cell activation the authors studied the effect of altering calcium availability for B cells stimulated by different classes of mitogens. 1mM EGTA inhibited B cell thymidine incorporation stimulated by LPS, 8-mercaptoguanosine (8sGuo) or anti-Ig antibodies, but had no effect on cell viability in the culture system used. The slow phase calcium channel blocker verapamil suppressed /sup 3/H-thymidine incorporation to a lesser degree. B cell sIa expression was used as an indicator of an earlier B cell activation event. The calcium ionophore A23187 stimulated a dramatic increase in B cell sIa in a dose dependent manner which was inhibited in the presence of 1mM EGTA. This reduction in extracellular calcium only partially diminished the sIa augmentation resulting from anti-Ig antibody, and had no effect on that stimulated by LPS or 8sGuo. These findings suggest that extracellular calcium is required for later events in B cells activation while earlier events may be primarily dependent upon intracellular calcium stores. Furthermore, membrane Ig mediated B cell activation may require higher concentrations of intracellular calcium than other B cell activation pathways.

• Research Organization: WRAMC, Washington, DC
• OSTI ID: 5508555
• Report Number(s): CONF-8604222-; TRN: 86-026589
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CALCIUM
BIOLOGICAL AVAILABILITY
LYMPHOCYTES
VIABILITY
ANTIBODIES
BIOLOGICAL PATHWAYS
CELL PROLIFERATION
DOSE-RESPONSE RELATIONSHIPS
EGTA
GUANOSINE
MITOGENS
MOLECULES
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALCOHOLS
ALKALINE EARTH METALS
ANIMAL CELLS
AROMATICS
AZAARENES
AZINES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHELATING AGENTS
CONNECTIVE TISSUE CELLS
ELEMENTS
GLYCOLS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LEUKOCYTES
MATERIALS
METALS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PURINES
PYRIMIDINES
RIBOSIDES
SOMATIC CELLS
550201* - Biochemistry- Tracer Techniques