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Title: Lysis of typhus-group rickettsia-infected targets by lymphokine activated killers

The authors recently described a subset of OKT8, OKT3-positive lymphocytes from typhus-group rickettsia immune individuals which were capable of lysing autologous PHA-blasts or Epstein-Barr virus transformed B cells (LCL) infected with typhus-group rickettsiae. In order to determine if killing by these effectors was HLA-restricted, they stimulated peripheral blood mononuclear cells (PBMC) from typhus-group rickettsia immune individuals in vitro with typhus-group rickettsia-derived antigen for one week and then measured lysis of autologous LCL or HLA-mismatched LCL in a 4-6 hour Cr/sup 51/-release assay. There was significant lysis of both the autologous and the HLA-mismatched infected targets as compared to the corresponding uninfected targets. Since this suggested that the effectors were lymphokine activated killers (LAK) rather than cytotoxic T lymphocytes, they then tested this hypothesis by stimulating PBMC from both immune and non-immune individuals in vitro for one week with purified interleukin 2 and measuring lysis of infected, autologous LCL. PBMC thus treated, from both immune and non-immune individuals, were capable of significantly lysing autologous, infected LCL as compared to the non-infected control. They therefore conclude that targets infected with typhus-group rickettsiae are susceptible to lysis to LAK.
Authors:
;
Publication Date:
OSTI Identifier:
5506465
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA; TRN: 86-026730
Resource Type:
Conference
Resource Relation:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States); Journal Volume: 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Research Org:
Naval Medical Research Institute, Bethesda, MD
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; BLOOD CELLS; LYSIS; LYMPHOCYTES; CELL KILLING; RICKETTSIAE; INFECTIVITY; ANTIGENS; BIOLOGICAL FUNCTIONS; CHROMIUM 51; IMMUNE REACTIONS; LYMPHOKINES; TRACER TECHNIQUES; TYPHUS; ANIMAL CELLS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BODY FLUIDS; CHROMIUM ISOTOPES; CONNECTIVE TISSUE CELLS; DISEASES; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; FUNCTIONS; GROWTH FACTORS; INFECTIOUS DISEASES; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LEUKOCYTES; MATERIALS; MICROORGANISMS; MITOGENS; NUCLEI; ORGANIC COMPOUNDS; PROTEINS; RADIOISOTOPES; RICKETTSIAL DISEASES; SOMATIC CELLS 550901* -- Pathology-- Tracer Techniques