Glucose and amino acid metabolism in rat brain during sustained hypoglycemia

Wong, K L; Tyce, G M

doi:10.1007/BF00965097

Title: Glucose and amino acid metabolism in rat brain during sustained hypoglycemia

Full Record
Other Related Research

Abstract

The metabolism of glucose in brains during sustained hypoglycemia was studied. (U-/sup 14/C)Glucose (20 microCi) was injected into control rats, and into rats at 2.5 hr after a bolus injection of 2 units of insulin followed by a continuous infusion of 0.2 units/100 g rat/hr. This regimen of insulin injection was found to result in steady-state plasma glucose levels between 2.5 and 3.5 mumol per ml. In the brains of control rats carbon was transferred rapidly from glucose to glutamate, glutamine, gamma-aminobutyric acid and aspartate and this carbon was retained in the amino acids for at least 60 min. In the brains of hypoglycemic rats, the conversion of carbon from glucose to amino acids was increased in the first 15 min after injection. After 15 min, the specific activity of the amino acids decreased in insulin-treated rats but not in the controls. The concentrations of alanine, glutamate, and gamma-amino-butyric acid decreased, and the concentration of aspartate increased, in the brains of the hypoglycemic rats. The concentration of pyridoxal-5'-phosphate, a cofactor in many of the reactions whereby these amino acids are formed from tricarboxylic acid cycle intermediates, was less in the insulin-treated rats than in the controls. These data provide evidencemore »« less

Authors:: Wong, K L; Tyce, G M

Publication Date:: Fri Apr 01 00:00:00 EST 1983

Research Org.:: Faculty of Medicine, University of Calgary, Canada

OSTI Identifier:: 5505137

Resource Type:: Journal Article

Journal Name:: Neurochem. Res.; (United States)

Additional Journal Information:: Journal Volume: 8:4; Journal Issue: 4

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; ALANINES; METABOLISM; AMINOBUTYRIC ACID; GLUCOSE; GLUTAMINE; BLOOD CHEMISTRY; BRAIN; CARBON 14 COMPOUNDS; NUTRITIONAL DEFICIENCY; RATS; TRACER TECHNIQUES; ALDEHYDES; AMIDES; AMINO ACIDS; ANIMALS; AUTONOMIC NERVOUS SYSTEM AGENTS; BODY; CARBOHYDRATES; CARBOXYLIC ACIDS; CENTRAL NERVOUS SYSTEM; DRUGS; HEXOSES; ISOTOPE APPLICATIONS; LABELLED COMPOUNDS; MAMMALS; MONOSACCHARIDES; NERVOUS SYSTEM; NEUROREGULATORS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; ORGANS; RODENTS; SACCHARIDES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats


                    Wong, K L, and Tyce, G M. Glucose and amino acid metabolism in rat brain during sustained hypoglycemia.  United States: N. p., 1983. 
        Web.  doi:10.1007/BF00965097.

Copy to clipboard


                    Wong, K L, & Tyce, G M. Glucose and amino acid metabolism in rat brain during sustained hypoglycemia.  United States.  https://doi.org/10.1007/BF00965097

Copy to clipboard


                    Wong, K L, and Tyce, G M. 1983.  
        "Glucose and amino acid metabolism in rat brain during sustained hypoglycemia".  United States.  https://doi.org/10.1007/BF00965097.

Copy to clipboard


                    
@article{osti_5505137,

  title        = {Glucose and amino acid metabolism in rat brain during sustained hypoglycemia},

  author       = {Wong, K L and Tyce, G M},

  abstractNote = {The metabolism of glucose in brains during sustained hypoglycemia was studied. (U-/sup 14/C)Glucose (20 microCi) was injected into control rats, and into rats at 2.5 hr after a bolus injection of 2 units of insulin followed by a continuous infusion of 0.2 units/100 g rat/hr. This regimen of insulin injection was found to result in steady-state plasma glucose levels between 2.5 and 3.5 mumol per ml. In the brains of control rats carbon was transferred rapidly from glucose to glutamate, glutamine, gamma-aminobutyric acid and aspartate and this carbon was retained in the amino acids for at least 60 min. In the brains of hypoglycemic rats, the conversion of carbon from glucose to amino acids was increased in the first 15 min after injection. After 15 min, the specific activity of the amino acids decreased in insulin-treated rats but not in the controls. The concentrations of alanine, glutamate, and gamma-amino-butyric acid decreased, and the concentration of aspartate increased, in the brains of the hypoglycemic rats. The concentration of pyridoxal-5'-phosphate, a cofactor in many of the reactions whereby these amino acids are formed from tricarboxylic acid cycle intermediates, was less in the insulin-treated rats than in the controls. These data provide evidence that glutamate, glutamine, aspartate, and GABA can serve as energy sources in brain during insulin-induced hypoglycemia.},

  doi          = {10.1007/BF00965097},

  url          = {https://www.osti.gov/biblio/5505137},
  journal      = {Neurochem. Res.; (United States)},
number       = 4,

  volume       = 8:4,

  place        = {United States},

  year         = {Fri Apr 01 00:00:00 EST 1983},

  month        = {Fri Apr 01 00:00:00 EST 1983}

}

Copy to clipboard

Journal Article:

https://doi.org/10.1007/BF00965097

Other availability

Find in Google Scholar

Search WorldCat to find libraries that may hold this journal

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

The effect of inhibition of hexose-monophosphate shunt on the metabolism of glucose and function in rat brain in vivo

Journal Article Gaitonde, M; Evans, G - Neurochem. Res.; (United States)

Rats treated 4 hr previously with 6-aminonicotinamide showed a twenty-four fold increase of (/sup 14/C)phosphogluconate in the adult brain at 30 min after intection of (U-/sup 14/C)glucose indicating a blockade of the hexosemmonophosphate shunt. There was a significant increase in the /sup 14/C-content of glucose and glucose-6-phosphate, and a decrease in that of amino acids. (/sup 14/C)Phosphoglycerate content showed no consistent change after 6-aminonicotinamide treatment. The concentration of glucose and glucose 6-phosphate increased significantly without a significant change in the lactate pool in the brain of 6-aminonicotinamide treated rats. The rate of utilization of glucose in the brain of controlmore »« less
https://doi.org/10.1007/BF00964893
A proposed method for the determination of cerebral regional intermediary glucose metabolism in humans in vivo using specifically labeled /sup 11/C-glucose and positron emission transverse tomography (PETT). I. An animal model with /sup 14/C-glucose and rat brain autoradiography

Journal Article Sacks, W; Sacks, S; Badalamenti, A; ... - J. Neurosci. Res.; (United States)

Based upon data obtained with our arterio-venous technique for the determination of cerebral metabolism in humans in vivo we have proposed a method for the determination of cerebral regional intermediary glucose metabolism in humans in vivo using specifically labeled /sup 11/C-glucose and positron emission transverse tomography (PETT). In it we would give the subject successive intravenous injections of (3,4-/sup 11/C) glucose, (2,5-/sup 11/C) glucose and (1-/sup 11/C) glucose. There would be a 30 min period of continuous PETT measurements following each injection and a 2 hr interval after the first and second injections. The data would be used with suitablemore »« less
https://doi.org/10.1002/jnr.490070107
Change in hexose distribution volume and fractional utilization of ( sup 18 F)-2-deoxy-2-fluoro-D-glucose in brain during acute hypoglycemia in humans

Journal Article Shapiro, E; Cooper, M; Chen, C; ... - Diabetes; (USA)

We used positron emission tomography (PET) to study the effects of mild hypoglycemia on cerebral glucose uptake and metabolism. Nine healthy men were studied under basal saline-infusion conditions, and during euglycemic and hypoglycemic clamp studies. Insulin was infused at the same rate (1 mU.kg-1.min-1) in both clamp studies. In euglycemic clamp studies, glucose was infused at a rate sufficient to maintain the basal plasma glucose concentration, whereas in hypoglycemic clamp studies, the glucose infusion rate was reduced to maintain the plasma glucose at 3.1 mM. Each study lasted 3 h and included a 30-min baseline period and a subsequent 150-minmore »« less
https://doi.org/10.2337/diab.39.2.175
The effect of 6-aminonicotinamide on the levels of brain amino acids and glucose, and their labeling with 14C after injection of (U-14C) glucose

Journal Article Gaitonde, M; Lewis, L; Evans, G; ... - Neurochem. Res.; (United States)

The brains of rats paralysed at 4 hr after the administration of 6-aminonicotinamide were found to contain decreased levels of glutamate and gamma-aminobutyrate. The glucose content of the brain of the treated rats was several fold higher than in controls. The incorporation of 14C into brain amino acids at 30 min after the injection of (U-14C)glucose was decreased by 16%: this was attributed to mainly decreased labeling of glutamate and associated amino acids. The results are discussed in the light of previous findings that the administration of 6-aminonicotinamide resulted in the blockade of the direct oxidation of glucose by themore » pentose phosphate pathway.« less
https://doi.org/10.1007/BF00964420
beta. -Receptor-mediated increase in cerebral blood flow during hypoglycemia

Journal Article Hollinger, B; Bryan, R - American Journal of Physiology; (USA)

The authors tested the hypothesis that {beta}-adrenergic receptor stimulation is involved with the increase in regional cerebral blood flow (rCBF) during hypoglycemia. Rats were surgically prepared with the use of halothane-nitrous oxide anesthesia. A plaster restraining cast was placed around the hindquarters, and anesthesia was discontinued. Hypoglycemia was produced by an intravenous injection of insulin; normoglycemic control rates were given saline. Propranolol was administered to some control and some hypoglycemic rats to block the {beta}-adrenergic receptors. Regional CBF was measured using 4-(N-methyl-{sup 14}C)iodoantipyrine. Regional CBF increased during hypoglycemia in rats that were not treated with propranolol. The increase varied frommore »« less

Similar Records