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Title: Increased levels of peroxisomal active oxygen-related enzymes in copper-tolerant pea plants

Abstract

The effect in vivo of high nutrient levels of copper (240 micromolar) on the activity of different metalloenzymes containing Cu, Mn, Fe, and Zn, distributed in chloroplasts, peroxisomes, and mitochondria, was studied in leaves of two varieties of Pisum sativum L. plants with different sensitivity to copper. The metalloenzymes studied were: cytochrome c oxidase, Mn-superoxide dismutase (Mn-SOD) and Cu,Zn-superoxide dismutase I (Cu,Zn-SOD I), for mitochondria; catalase and Mn-SOD, for peroxisomes; and isozyme Cu,Zn-SOD II for chloroplasts. The activity of mitochondrial SOD isozymes (Mn-SOD and Cu,Zn-SOD I) was very similar in Cu-tolerant and Cu-sensitive plants, whereas cytochrome c oxidase was lower in Cu-sensitive plants. Chloroplastid Cu,Zn-SOD activity was the same in the two plant varieties. In contrast, the peroxisomal Mn-SOD activity was considerably higher in Cu-tolerant than in Cu-sensitive plants, and the activity of catalase was also increased in peroxisomes of Cu-tolerant plants. The higher activities of these peroxisomal active oxygen-related enzymes in Cu-tolerant plants suggest the involvement of reactive oxygen intermediates (O/sub 2//sup -/, OH) in the mechanism of Cu lethality, and also imply a function for peroxisomal Mn-SOD in the molecular mechanisms of plant tolerance to Cu in Pisum sativum L.

Authors:
; ; ;
Publication Date:
Research Org.:
Consejo Superior de Investigaciones Cientificas, Granada, Spain
OSTI Identifier:
5504238
Resource Type:
Journal Article
Journal Name:
Plant Physiol.; (United States)
Additional Journal Information:
Journal Volume: 85:2
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CATALASE; ENZYME ACTIVITY; COPPER; TOXICITY; OXIDOREDUCTASES; PISUM; SENSITIVITY; SUPEROXIDE DISMUTASE; CHLOROPLASTS; GENETIC VARIABILITY; LEAVES; MITOCHONDRIA; BACTERIA; BIOLOGICAL VARIABILITY; CELL CONSTITUENTS; ELEMENTS; ENZYMES; LEGUMINOSAE; METALS; MICROORGANISMS; ORGANOIDS; PEROXIDASES; PLANTS; RHIZOBIUM; TRANSITION ELEMENTS; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Palma, J M, Gomez, M, Yanez, J, and Del Rio, L A. Increased levels of peroxisomal active oxygen-related enzymes in copper-tolerant pea plants. United States: N. p., 1987. Web. doi:10.1104/pp.85.2.570.
Palma, J M, Gomez, M, Yanez, J, & Del Rio, L A. Increased levels of peroxisomal active oxygen-related enzymes in copper-tolerant pea plants. United States. https://doi.org/10.1104/pp.85.2.570
Palma, J M, Gomez, M, Yanez, J, and Del Rio, L A. 1987. "Increased levels of peroxisomal active oxygen-related enzymes in copper-tolerant pea plants". United States. https://doi.org/10.1104/pp.85.2.570.
@article{osti_5504238,
title = {Increased levels of peroxisomal active oxygen-related enzymes in copper-tolerant pea plants},
author = {Palma, J M and Gomez, M and Yanez, J and Del Rio, L A},
abstractNote = {The effect in vivo of high nutrient levels of copper (240 micromolar) on the activity of different metalloenzymes containing Cu, Mn, Fe, and Zn, distributed in chloroplasts, peroxisomes, and mitochondria, was studied in leaves of two varieties of Pisum sativum L. plants with different sensitivity to copper. The metalloenzymes studied were: cytochrome c oxidase, Mn-superoxide dismutase (Mn-SOD) and Cu,Zn-superoxide dismutase I (Cu,Zn-SOD I), for mitochondria; catalase and Mn-SOD, for peroxisomes; and isozyme Cu,Zn-SOD II for chloroplasts. The activity of mitochondrial SOD isozymes (Mn-SOD and Cu,Zn-SOD I) was very similar in Cu-tolerant and Cu-sensitive plants, whereas cytochrome c oxidase was lower in Cu-sensitive plants. Chloroplastid Cu,Zn-SOD activity was the same in the two plant varieties. In contrast, the peroxisomal Mn-SOD activity was considerably higher in Cu-tolerant than in Cu-sensitive plants, and the activity of catalase was also increased in peroxisomes of Cu-tolerant plants. The higher activities of these peroxisomal active oxygen-related enzymes in Cu-tolerant plants suggest the involvement of reactive oxygen intermediates (O/sub 2//sup -/, OH) in the mechanism of Cu lethality, and also imply a function for peroxisomal Mn-SOD in the molecular mechanisms of plant tolerance to Cu in Pisum sativum L.},
doi = {10.1104/pp.85.2.570},
url = {https://www.osti.gov/biblio/5504238}, journal = {Plant Physiol.; (United States)},
number = ,
volume = 85:2,
place = {United States},
year = {Thu Oct 01 00:00:00 EDT 1987},
month = {Thu Oct 01 00:00:00 EDT 1987}
}