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Title: Phorbol esters alter the expression of lymphocyte membrane proteins

T cell activation via the T cell receptor (T3-Ti complex) by OKT3 results in modulation of the T3-Ti complex, but does not affect T4, T8, or T11 antigen expression. To study the effect of other T cell activators on these T cell membrane antigens, the authors incubated mononuclear cells for 0-3 days with lectins or pharmacologic agents and stained with monoclonal antibodies to their antigens. The median fluorescence intensity (MFI) was measured with a fluorescence activated cell sorter. Activation of PBL with Con A, PHA, calcium ionophore A23187, or with dbcAMP, isoproterenol, or theophyllin had minimal effects on the MFI of T3, T4, T8, or T11. Phorbol myristate acetate (PMA), a protein kinase C activator which stimulates PBL though an alternate pathway, caused a 90-100% reduction of T3 and T4 MFI, a 25% reduction in T8 MFI, and a 400% increase in T11 MFI after 2 days. Addition of A23187 slightly increased these effects. PMA induced a 2-3-fold increase in cell diameter concomitant with the alterations in membrane antigens. These data suggest that T cell activation through pathways not directly linked to the T cell antigen receptor can result in surface antigen expression different from that which follows activation viamore » the T cell receptor.« less
Authors:
;
Publication Date:
OSTI Identifier:
5499363
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA
Resource Type:
Conference
Resource Relation:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States); Journal Volume: 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Research Org:
Univ. of Chicago, IL
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; LYMPHOCYTES; RECEPTORS; PHORBOL ESTERS; BIOLOGICAL EFFECTS; AMP; ANTIGENS; CELL MEMBRANES; CONCANAVALIN; FLUORESCENCE; LECTINS; MONOCLONAL ANTIBODIES; PHYTOHEMAGGLUTININ; AGGLUTININS; ANIMAL CELLS; ANTIBODIES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOHYDRATES; CARCINOGENS; CELL CONSTITUENTS; CONNECTIVE TISSUE CELLS; ESTERS; HEMAGGLUTININS; LEUKOCYTES; LUMINESCENCE; MATERIALS; MEMBRANE PROTEINS; MEMBRANES; MITOGENS; MUCOPROTEINS; NUCLEOTIDES; ORGANIC COMPOUNDS; POLYSACCHARIDES; PROTEINS; SACCHARIDES; SOMATIC CELLS 560301* -- Chemicals Metabolism & Toxicology-- Cells-- (-1987)