Inhibition by probenecid of 2,3-dimercaptopropane sulfonate (DMPS) induced excretion of mercury from the isolated perfused rat kidney (IPRK)

Conference · Sat Mar 01 00:00:00 EST 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5498258

Klotzbach, J M; Diamond, G L

DMPS is a complexing agent that is being considered for use in the treatment of heavy metal poisoning. In rats, DMPS undergoes renal tubular secretion by a probenecid (PRB) sensitive mechanism. The goal of this study was to determine the role of tubular secretion in the DMPS-induced excretion of mercury from the kidneys. Male Long-Evans rats were injected with HgCl/sub 2/ (100 ..mu..g Hg/kg, ip) and 48 hr later were perfused for 30 min at 38/sup 0/C with a Krebs-Ringer bicarbonate solution containing 4.5% polyvinylpyrrolidone, a mixture of metabolic substrates, inulin (0.8 mg/ml) and DMPS (23 ..mu..M). The renal excretory clearance of ultrafilterable DMPS (CD) exceeded the simultaneously measured clearance of inulin (CI); DMPS-to-inulin clearance ratio (CD/CI) = 6.06 +/- 1.80 (n = 3), indicating net tubular secretion of DMPS. Under these conditions, 26.8 +/- 4.6% (n = 4) of the Hg burden was removed from the kidneys and excreted exclusively in the urine. In the absence of DMPS, no Hg was removed from the kidney. PRB (230 ..mu..M) completely inhibited net tubular secretion of DMPS (CD/CI = 0.87 +/- 0.07, n = 3) and in the presence of PRB and DMPS, only 3.2 +/- 1.0% (n = 4) of the renal Hg burden was removed and excreted in urine. Thus, tubular secretion of DMPS is important for the DMPS-induced excretion of Hg from the kidney.

• Research Organization: Univ. of Rochester, NY
• OSTI ID: 5498258
• Report Number(s): CONF-8604222-
• Journal Information: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BENZOIC ACID
BIOLOGICAL EFFECTS
MERCURY
EXCRETION
SULFONATES
INHIBITION
RENAL CLEARANCE
INULIN
KIDNEYS
PERFUSED ORGANS
RATS
TUBULES
ANIMALS
BODY
CARBOHYDRATES
CARBOXYLIC ACIDS
CLEARANCE
ELEMENTS
MAMMALS
METALS
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
POLYSACCHARIDES
RODENTS
SACCHARIDES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)