Isolation and characterization of a methylotrophic marine methanogen, Methanococcoides methylutens gen. nov. , sp

Conference · Tue Feb 01 00:00:00 EST 1983 · Appl. Environ. Microbiol.; (United States)

OSTI ID:5495449

Sowers, K R; Ferry, J G

A new genus of marine methanogenic bacteria is described that utilizes trimethylamine, diethylamine, monomethylamine, and methanol as substrates for growth and methanogenesis. Methane was not produced from H/sub 2/-CO/sub 2/, sodium formate, or sodium acetate. Growth on trimethylamine was stimulated by yeast extract, Trypticase (BBL Microbiology Systems, Cockeysville, MD), rumen fluid, or B vitamins. The optimal growth temperature was 30 to 35/sup 0/C. The maximum growth rate was between pH 7.0 and 7.5. Na/sup +/ (0.4 M) and MgSO/sub 4/ (0.05 M) were required for maximum growth. Colonies of the type strain, TMA-10, were yellow, circular, and convex with entire edges. Cells were nonmotile, nonsporeforming, irregular cocci 1 ..mu..m in diameter which stained gram negative and occurred singly or in pairs. Micrographs of thin sections revealed a monolayered cell wall approximately 10-nm thick which consisted of protein. Cells were lysed in 0.01% sodium dodecyl sulfate or 0.001% Triton X-100. The DNA base composition was 42 mol% guanine plus cytosine. Methanococcoides is the proposed genus and Methanococcoides methylutens is the type species. TMA-10 is the type strain (ATCC 33938).

• Research Organization: Virginia Polytechnic Inst. and State Univ., Blacksburg
• OSTI ID: 5495449
• Journal Information: Appl. Environ. Microbiol.; (United States), Vol. 45:2; Conference: 82nd Annual Meeting of the American Society for Microbiology, 1982
• Country of Publication: United States
• Language: English

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AMINO ACIDS
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