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Title: Inhibition of human natural killer (NK) cytotoxicity by Candida albicans

Experiments were initiated to determine whether human NK cells are cytotoxic to C. albicans with similar activity observed for mouse NK cells against the yeast Paracoccidiodes brasiliensis. In 48 hour assays using limiting dilutions of C. albicans, strain 3153A, mononuclear leukocytes with NK activity had only marginal effects on yeast outgrowth, whereas granulocytes killed most of the yeast. However, these yeast were able to block NK activity in 4 hr /sup 51/Cr release assays with K562 cells, at yeast to K562 ratios of 10:1 and 100:1. Yeast pretreated with the serum of the majority of donors blocked the NK activity more than untreated yeast. Two of the 7 donors did not enhance NK inhibition after pretreatment of the yeast with their serum. Serum antibody to C. albicans and complement consumption by the yeast correlated with the relative efficiency of NK inhibition for most donors. This report suggests that there may be in vivo interactions between NK cells of the immune system and opportunistic fungal pathogens, which may compromise NK cell function.
Authors:
;
Publication Date:
OSTI Identifier:
5495214
Report Number(s):
CONF-8604222-
Journal ID: CODEN: FEPRA; TRN: 86-026756
Resource Type:
Conference
Resource Relation:
Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States); Journal Volume: 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
Research Org:
Univ. of Nevada, Reno
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; CANDIDA; CELL KILLING; LYMPHOCYTES; INHIBITION; ANTIBODIES; BIOLOGICAL FUNCTIONS; CHROMIUM 51; COMPLEMENT; MAN; MICE; RETICULOENDOTHELIAL SYSTEM; TRACER TECHNIQUES; YEASTS; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CHROMIUM ISOTOPES; CONNECTIVE TISSUE CELLS; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; FUNCTIONS; FUNGI; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; LEUKOCYTES; MAMMALS; MATERIALS; MICROORGANISMS; NUCLEI; PLANTS; PRIMATES; RADIOISOTOPES; RODENTS; SOMATIC CELLS; TISSUES; VERTEBRATES 551001* -- Physiological Systems-- Tracer Techniques