Increased osmolarity with low molecular weight solutes inhibits CTL-mediated cytotoxicity
Sucrose induced hyperosmolarity, which has no effect on complement-mediated lysis, was examined for its effect on CTL-mediated cell lysis. Sucrose (0.15M) inhibited H-2/sup b/ anti-H-2/sup d/ PEL specific lysis, as measured by /sup 51/Cr-release, 82.7% (52 +/-1.4 vs. 9 +/- 0.6). The effect of sucrose on the killer cell independent phase was investigated by allowing the killers and targets to interact for 10 minutes at 37/sup 0/C, and then inactivating the killers by a 5 minute incubation at 44/sup 0/C before adjusting the osmolarity. Under these conditions killing was reduced 65.5% (58 +/- 0.5 vs. 20 +/- 0.8) when hit P815 target cells were incubated in 0.15M sucrose; thus indicating inhibition was at the level of the target cell. This effect was not limited to sucrose as Na/sub 2/SO/sub 4/ (.08M) also inhibited /sup 51/Cr-release. Nuclear fragmentation as indicated by release of incorporated /sup 3/H-thymidine also was inhibited in the presence of high osmolarity, although to a lesser extent, 34% (47 +/- 1.6 vs 31 +/- 0.6) after 40 minutes. These data suggest a surprising difference between poly C9 and CTL polyperforins, in light of their reported homologies.
- Research Organization:
- Univ. of Connecticut Health Center, Farmington
- OSTI ID:
- 5495207
- Report Number(s):
- CONF-8604222-; TRN: 86-026757
- Journal Information:
- Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States), Vol. 45:3; Conference: 70. annual meeting of the Federation of American Society for Experimental Biology, St. Louis, MO, USA, 13 Apr 1986
- Country of Publication:
- United States
- Language:
- English
Similar Records
Complement bound to tumor target cells enhances their sensitivity to macrophage-mediated killing
Virus-specific HLA-restricted lysis of herpes simplex virus-infected human monocytes and macrophages mediated by cytotoxic T lymphocytes
Related Subjects
LYMPHOCYTES
INHIBITION
SACCHAROSE
BIOLOGICAL EFFECTS
SODIUM SULFATES
CELL KILLING
CHROMIUM 51
CLONE CELLS
LYSIS
MOLECULAR WEIGHT
THYMIDINE
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALI METAL COMPOUNDS
ANIMAL CELLS
AZINES
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOHYDRATES
CELL CULTURES
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DISACCHARIDES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
LEUKOCYTES
MATERIALS
NUCLEI
NUCLEOSIDES
NUCLEOTIDES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PYRIMIDINES
RADIOISOTOPES
RIBOSIDES
SACCHARIDES
SODIUM COMPOUNDS
SOMATIC CELLS
SULFATES
SULFUR COMPOUNDS
551001* - Physiological Systems- Tracer Techniques