Comparison of S9 mix and hepatocytes as external metabolizing systems in mammalian cell cultures: cytogenetic effects of 7,12-dimethylbenzanthracene and aflatoxin B1
Two external metabolizing systems, S9 mix from Aroclor-induced rat livers and freshly isolated hepatocytes, were used for activation in cultures of human lymphocytes and V79 cells. 7, 12-dimethylbenzanthracene (DMBA) and aflatoxin B1 (AFB1) were employed as indirectly acting reference mutagens. Mutagenic effects were measured by induction of sister chromatid exchange (SCE). With DMBA, SCE-inducing effects were found to be quite similar after activation by S9 mix and activation by hepatocytes. In contrast with AFB1, S9 activation led to a stronger SCE induction than hepatocyte activation in both target cells. The induction of chromosomal aberrations by AFB1 after activation by the two metabolizing systems was also analyzed in V79 cells. This experiment again revealed that AFB1 was more efficiently activated by S9 mix than by hepatocytes. The experiments have shown that the suitability of hepatocytes as an activation system is not restricted to microbial or eukaryotic point mutation assays, but that hepatocyte metabolism can also be successfully included in cytogenetic tests with short- and long-term cultures of mammalian target cells.
- Research Organization:
- Bundesgesundheitsamt, Berlin, West Germany
- OSTI ID:
- 5493964
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 8:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
AFLATOXIN
GENETIC EFFECTS
METABOLIC ACTIVATION
DIMETHYLBENZANTHRACENE
DOSE-RESPONSE RELATIONSHIPS
FIBROBLASTS
LIVER CELLS
LYMPHOCYTES
RATS
SISTER CHROMATID EXCHANGES
ANIMAL CELLS
ANIMALS
ANTIGENS
AROMATICS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMOSOMAL ABERRATIONS
CONDENSED AROMATICS
CONNECTIVE TISSUE CELLS
LEUKOCYTES
MAMMALS
MATERIALS
MUTATIONS
ORGANIC COMPOUNDS
RODENTS
SOMATIC CELLS
TOXIC MATERIALS
TOXINS
VERTEBRATES
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)