Distribution in cerebrospinal fluid, blood, and lymph of epidurally injected morphine and inulin in dogs
We describe procedures for catheterizing the epidural space, the azygos vein, and the thoracic lymph duct of dogs without using fluoroscopy. The success rates of the procedures were 100, 80, and 50%, respectively (n = 10). To assess the validity of the model, /sup 3/H-morphine and unlabeled morphine (2 mg) were injected epidurally in ten dogs. Lumbar cerebrospinal fluid (CSF), azygos venous blood, arterial blood, and lymph were sampled before and 5, 20, 60, 120, 180, 240, 300 and 360 min after injection. During the first 20 min, morphine levels in the azygos vein were about three and ten times greater than arterial and lymphatic levels, respectively (n = 3; P less than 0.01). Morphine levels were significantly greater in the azygos vein (n = 8) and the femoral artery (n = 10) during the first 20 and 60 min than they were later, respectively (P less than 0.05). In the lymph (n = 5), the levels of morphine at 60 min were statistically greater (P less than 0.05) than levels at 4, 5, and 6 hr. At no time were the concurrent arterial and lymph levels different from each other. In the lumbar CSF, the morphine peak concentration was reached 5-60 min after epidural injection and ranged between 5 and 93 micrograms/ml. In the CSF, the levels of morphine were significantly greater during the first 20 min than later (n = 7; P less than 0.05). The washout of the lumbar CSF curve for morphine appeared to be fitted by a two-compartment open model. The t1/2-alpha and t1/2-beta values were 14.7 +/- 7.2 min and 106 +/- 45 min, respectively (mean +/- SD). Cumulative percentages of the epidural dose of morphine passed into the azygos system within the first 5, 20, 60, and 120 min after injection were calculated to be 4.0 +/- 2.1, 23.5 +/- 14.6, 49.2 +/- 34.2, and 55.9 +/- 35.3, respectively (mean +/- SD; n = 8).
- Research Organization:
- Mayo Clinic, Rochester, MN
- OSTI ID:
- 5491072
- Journal Information:
- Anesth. Analg. (Cleveland); (United States), Vol. 6
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
INULIN
METABOLISM
TISSUE DISTRIBUTION
MORPHINE
BIOCHEMICAL REACTION KINETICS
BIOLOGICAL HALF-LIFE
BIOLOGICAL MODELS
BLOOD
CEREBROSPINAL FLUID
DOGS
LYMPH
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALOIDS
ANALGESICS
ANIMALS
BIOLOGICAL MATERIALS
BODY FLUIDS
CARBOHYDRATES
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DISTRIBUTION
DRUGS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
MATERIALS
NARCOTICS
OPIUM
ORGANIC COMPOUNDS
POLYSACCHARIDES
REACTION KINETICS
SACCHARIDES
VERTEBRATES
550501* - Metabolism- Tracer Techniques