Preparative-scale proteins seperations by continuous annular chromatography

Conference · Sun Jan 01 00:00:00 EST 1989

OSTI ID:5483357

Bloomingburg, G F; Bauer, J S; Carta, G; Byers, C H ^[1]

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1. Virginia Univ., Charlottesville, VA (USA). Dept. of Chemical Engineering

The use of continuous annular chromatography (CAC) for the separation of protein mixtures is studied in a preparative-scale CAC unit. S-Sepharose, a strong-acid porous cation exchange resin is used as the separation medium mixtures of albumin, hemoglobin, and cytochrome-C are used as a model separation system. Equilibrium and mass transfer parameter are developed for this system on the basis of fixed-bed chromatograph experiments. A mathematical model is then successfully used in conjunction with these parameters to simulate the performance of the CAC separations. 11 refs., 11 figs., 3 tabs.

• Research Organization: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
• Sponsoring Organization: DOE/CE
• DOE Contract Number: AC05-84OR21400
• OSTI ID: 5483357
• Report Number(s): CONF-891013-3; ON: DE90001954
• Resource Relation: Conference: 6. symposium on separation science and technology for energy applications, Knoxville, TN (USA), 22-27 Oct 1989
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
PROTEINS
CHROMATOGRAPHY
SEPARATION PROCESSES
MATHEMATICAL MODELS
ION EXCHANGE
ION EXCHANGE MATERIALS
RESINS
SEPARATION EQUIPMENT
MATERIALS
ORGANIC COMPOUNDS
ORGANIC POLYMERS
PETROCHEMICALS
PETROLEUM PRODUCTS
POLYMERS
400202* - Isotope Effects
Isotope Exchange
& Isotope Separation