Notexin preferentially inhibits the release of newly synthesized acetylcholine from rat brain synaptosomal fractions

Journal Article · Thu Jan 01 00:00:00 EST 1981 · J. Neurosci. Res.; (United States)

OSTI ID:5479328

Gundersen, C B; Jenden, D J

An investigation was made of the effects of the snake venom neurotoxin, notexin, on acetylcholine turnover in rat brain P2 fractions using a gas chromatographic mass spectrometric assay for acetylcholine and choline. In contrast to earlier reports, we found a stimulation of the uptake and acetylation of labeled choline by toxin-treated P2 fractions. More significantly, notexin inhibited the release of this newly synthesized transmitter. These effects were found to be dependent on the dose of the toxin and the time of exposure of the P2 fraction to notexin. Longer exposure to notexin or experiments involving resuspension of notexin-treated P2 fractions appeared to result in considerable lysis of the transmitter-containing particles. Thus, notexin may alter acetylcholine compartmentation in the nerve ending and thereby affect acetylcholine synthesis.

• Research Organization: Department of Pharmacology, School of Medicine, University of California, Los Angeles
• OSTI ID: 5479328
• Journal Information: J. Neurosci. Res.; (United States), Vol. 10:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
BIOSYNTHESIS
GAS CHROMATOGRAPHY
MASS SPECTROSCOPY
CHLORINE
UPTAKE
DEUTERIUM COMPOUNDS
VENOMS
BIOLOGICAL EFFECTS
BRAIN
ISOTOPE DILUTION
RATS
SNAKES
AMINES
AMMONIUM COMPOUNDS
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BODY
CENTRAL NERVOUS SYSTEM
CHROMATOGRAPHY
DRUGS
ELEMENTS
ESTERS
HALOGENS
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
MAMMALS
NERVOUS SYSTEM
NEUROREGULATORS
NONMETALS
ORGANIC COMPOUNDS
ORGANS
PARASYMPATHOMIMETICS
QUATERNARY COMPOUNDS
REPTILES
RODENTS
SEPARATION PROCESSES
SPECTROSCOPY
SYNTHESIS
TRACER TECHNIQUES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques