Interactions of carcinogens with DNA (deoxyribonucleic acid)
The principal goal of this research has been the determination of the conformational changes produced in DNA by the covalent binding of a carcinogenic aromatic amine, and the correlation of these changes with the mutations and carcinogenic effects initiated by the same substances. To this end, we have devised new synthetic methods for the preparation of oligonucleotides modified by derivatives af 4-aminobiphenyl and aniline. We have also performed potential energy minimization studies on the above substances and on single and double stranded DNA fragments bearing the above amines as well as acetylaminofluorene, aminofluorene, aminopyrene and the antibiotic mitomycin. Our computations have been carried out on DOE supercomputers using our program, DUPLEX. We have defined a number of novel structures for these modified DNAs, including Hoogsteen, wedge'' (see below) denatured, cross-linked and intercalated forms. Some suggestions have been made about the relation of these forms to mutagenesis. 7 refs.
- Research Organization:
- New York Univ. (NYU), NY (United States)
- Sponsoring Organization:
- DOE/ER
- DOE Contract Number:
- AC02-81ER60015
- OSTI ID:
- 5477407
- Report Number(s):
- DOE/ER/60015-7; ON: DE90002880
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
CONFORMATIONAL CHANGES
MATHEMATICAL MODELS
DNA ADDUCTS
CHEMICAL PREPARATION
ANILINE
BENZOPYRENE
DICHROISM
DNA
HYPOTHESIS
NUCLEAR MAGNETIC RESONANCE
OLIGONUCLEOTIDES
POLYCYCLIC AROMATIC AMINES
PROGRESS REPORT
THERMODYNAMICS
ADDUCTS
AMINES
AROMATICS
CONDENSED AROMATICS
DOCUMENT TYPES
HYDROCARBONS
MAGNETIC RESONANCE
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RESONANCE
SYNTHESIS
560300* - Chemicals Metabolism & Toxicology
550200 - Biochemistry
400201 - Chemical & Physicochemical Properties