Inhibition of natural killer cell activity by eicosapentaenoic acid in vivo and in vitro

Journal Article · Fri Jan 15 00:00:00 EST 1988 · Biochem. Biophys. Res. Commun.; (United States)

DOI:https://doi.org/10.1016/0006-291X(88)90548-7· OSTI ID:5471684

Yamashita, N; Sugiyama, E; Hamazaki, T; Yano, S

To examine the effects of in vivo eicosapentaenoic acid (EPA) on natural killer (NK) cell activity, C3H/He mice each received a single intraperitoneal bolus of an emulsion of trieicosapentaenoyl-glycerol (EPA-TG). Spleen cells were tested for NK activity using /sup 51/Chromium-release assays against YAC-1 target cells. Forty eight hours after injection, NK activity was inhibited in a dose-dependent manner. EPA-TG emulsion also inhibited the NK activity of NK-enriched effector cells. Decreased cytotoxicity was first noted 24 hr after injection; it resumed the baseline by 7 days. The addition of EPA-TG emulsion to a cytotoxicity assay system resulted in moderate depression of NK activity. These results demonstrate that EPA has significant immunomodulatory effects on NK activity.

• Research Organization: Toyama Medical and Pharmaceutical Univ., Japan
• OSTI ID: 5471684
• Journal Information: Biochem. Biophys. Res. Commun.; (United States), Vol. 150:1
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
EICOSANOIC ACID
BIOCHEMICAL REACTION KINETICS
LYMPHOCYTES
CELL PROLIFERATION
CELL KILLING
CHROMIUM 51
DOSE-RESPONSE RELATIONSHIPS
EMULSIONS
IN VITRO
IN VIVO
INHIBITION
MICE
MONOCYTES
SPLEEN CELLS
TRACER TECHNIQUES
TRIGLYCERIDES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CHROMIUM ISOTOPES
COLLOIDS
CONNECTIVE TISSUE CELLS
DISPERSIONS
ELECTRON CAPTURE RADIOISOTOPES
ESTERS
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LEUKOCYTES
LIPIDS
MAMMALS
MATERIALS
MONOCARBOXYLIC ACIDS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
RADIOISOTOPES
REACTION KINETICS
RODENTS
SOMATIC CELLS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques