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Title: Inhibition of natural killer cell activity by eicosapentaenoic acid in vivo and in vitro

To examine the effects of in vivo eicosapentaenoic acid (EPA) on natural killer (NK) cell activity, C3H/He mice each received a single intraperitoneal bolus of an emulsion of trieicosapentaenoyl-glycerol (EPA-TG). Spleen cells were tested for NK activity using /sup 51/Chromium-release assays against YAC-1 target cells. Forty eight hours after injection, NK activity was inhibited in a dose-dependent manner. EPA-TG emulsion also inhibited the NK activity of NK-enriched effector cells. Decreased cytotoxicity was first noted 24 hr after injection; it resumed the baseline by 7 days. The addition of EPA-TG emulsion to a cytotoxicity assay system resulted in moderate depression of NK activity. These results demonstrate that EPA has significant immunomodulatory effects on NK activity.
Authors:
; ; ;
Publication Date:
OSTI Identifier:
5471684
Resource Type:
Journal Article
Resource Relation:
Journal Name: Biochem. Biophys. Res. Commun.; (United States); Journal Volume: 150:1
Research Org:
Toyama Medical and Pharmaceutical Univ., Japan
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; EICOSANOIC ACID; BIOCHEMICAL REACTION KINETICS; LYMPHOCYTES; CELL PROLIFERATION; CELL KILLING; CHROMIUM 51; DOSE-RESPONSE RELATIONSHIPS; EMULSIONS; IN VITRO; IN VIVO; INHIBITION; MICE; MONOCYTES; SPLEEN CELLS; TRACER TECHNIQUES; TRIGLYCERIDES; ANIMAL CELLS; ANIMALS; BETA DECAY RADIOISOTOPES; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY FLUIDS; CARBOXYLIC ACIDS; CHROMIUM ISOTOPES; COLLOIDS; CONNECTIVE TISSUE CELLS; DISPERSIONS; ELECTRON CAPTURE RADIOISOTOPES; ESTERS; EVEN-ODD NUCLEI; INTERMEDIATE MASS NUCLEI; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; LEUKOCYTES; LIPIDS; MAMMALS; MATERIALS; MONOCARBOXYLIC ACIDS; NUCLEI; ORGANIC ACIDS; ORGANIC COMPOUNDS; RADIOISOTOPES; REACTION KINETICS; RODENTS; SOMATIC CELLS; VERTEBRATES 550201* -- Biochemistry-- Tracer Techniques