Whole-cell bioconversion of vanillin to vanillic acid by Streptomyces viridosporus
Abstract
A two-step batch fermentation-bioconversion of vanillin (4-hydroxy-3-methoxybenzaldehyde) to vanillic acid (4-hydroxy-3-methoxybenzoic acid) was developed, utilizing whole cells of Streptomyces viridosporus T7A. In the first step, cells were grown in a yeast extract-vanillin medium under conditions where cells produced an aromatic aldehyde oxidase. In the second step, vanillin was incubated with the active cells and was quantitatively oxidized to vanillic acid which accumulated in the growth medium. Vanillic acid was readily recovered from the spent medium by a combination of acid precipitation and ether extraction at greater than or equal to96% molar yield and upon recrystallization from glacial acetic acid was obtained in greater than or equal to99% purity.
- Authors:
- Publication Date:
- Research Org.:
- Univ. of Idaho, Moscow
- OSTI Identifier:
- 5465538
- DOE Contract Number:
- AC02-80ER10755
- Resource Type:
- Journal Article
- Journal Name:
- Appl. Environ. Microbiol.; (United States)
- Additional Journal Information:
- Journal Volume: 45:5
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; ALDEHYDES; FERMENTATION; CARBOXYLIC ACIDS; BIOSYNTHESIS; BATCH CULTURE; STREPTOMYCES; BACTERIA; BIOCONVERSION; MICROORGANISMS; ORGANIC ACIDS; ORGANIC COMPOUNDS; SYNTHESIS; 550700* - Microbiology
Citation Formats
Pometto, III, A L, and Crawford, D L. Whole-cell bioconversion of vanillin to vanillic acid by Streptomyces viridosporus. United States: N. p., 1983.
Web.
Pometto, III, A L, & Crawford, D L. Whole-cell bioconversion of vanillin to vanillic acid by Streptomyces viridosporus. United States.
Pometto, III, A L, and Crawford, D L. 1983.
"Whole-cell bioconversion of vanillin to vanillic acid by Streptomyces viridosporus". United States.
@article{osti_5465538,
title = {Whole-cell bioconversion of vanillin to vanillic acid by Streptomyces viridosporus},
author = {Pometto, III, A L and Crawford, D L},
abstractNote = {A two-step batch fermentation-bioconversion of vanillin (4-hydroxy-3-methoxybenzaldehyde) to vanillic acid (4-hydroxy-3-methoxybenzoic acid) was developed, utilizing whole cells of Streptomyces viridosporus T7A. In the first step, cells were grown in a yeast extract-vanillin medium under conditions where cells produced an aromatic aldehyde oxidase. In the second step, vanillin was incubated with the active cells and was quantitatively oxidized to vanillic acid which accumulated in the growth medium. Vanillic acid was readily recovered from the spent medium by a combination of acid precipitation and ether extraction at greater than or equal to96% molar yield and upon recrystallization from glacial acetic acid was obtained in greater than or equal to99% purity.},
doi = {},
url = {https://www.osti.gov/biblio/5465538},
journal = {Appl. Environ. Microbiol.; (United States)},
number = ,
volume = 45:5,
place = {United States},
year = {Sun May 01 00:00:00 EDT 1983},
month = {Sun May 01 00:00:00 EDT 1983}
}