Relationship between number of spleen colonies and /sup 125/IdUrd incorporation into spleen and femur
- Tokyo Metropolitan Inst. of Gerontology, Japan
Graded numbers of bone marrow (BM) cells were injected into fatally irradiated mice. Eight days later the mice were given 3.0 ..mu..Ci (1 Ci = 3.7 x 10/sup 10/Bq) of /sup 125/IdUrd to label proliferating cells in the spleen and BM. On day 9 the mice were killed and the spleens and femurs were removed for splenic colony assay and measurement of radioactivity in the spleen and femurs. The number of splenic colonies shows a linear relationship with dose of marrow cells injected from 10/sup 4/ to 10/sup 5/ cells. The slope of the curve of spleen colonies versus number of cells injected is <1, implying that the fraction seeded in spleen decreases with number of cells injected. Above 10/sup 5/ and below 10/sup 4/ there is a striking departure from the simple linearity. Below 2 x 10/sup 3/ cells injected, the logarithm of the observed colony yield is linear with logarithm of the number of cells injected. Poisson calculation of the average number of pluripotent stem cells that should be present with numbers of marrow cells injected below 2 x 10/sup 3/ followed closely the actual observations. The data show that there is no detectible proliferation in the BM until the dose of marrow cells exceeds 3.5 x 10/sup 4/ cells. Induction of cells into cycle increases the seeding into the BM, and thymidine cytocide drastically reduces seeding in the BM, leading us to conclude that the BM is repopulated almost exclusively by stem cells in DNA synthesis.
- DOE Contract Number:
- AC02-76CH00016
- OSTI ID:
- 5464916
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 80:2
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BONE MARROW
STEM CELLS
RADIATION CHIMERAS
SPLEEN COLONY FORMATION
SPLEEN
LABELLING
BIOASSAY
BONE MARROW CELLS
DNA
DOSE-RESPONSE RELATIONSHIPS
FEMUR
INJECTION
LABELLED COMPOUNDS
LETHAL IRRADIATION
MICE
PRECURSOR
ANIMAL CELLS
ANIMAL TISSUES
ANIMALS
BODY
CHIMERAS
COLONY FORMATION
CONNECTIVE TISSUE CELLS
HEMATOPOIETIC SYSTEM
INTAKE
IRRADIATION
MAMMALS
MOSAICISM
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RODENTS
SKELETON
SOMATIC CELLS
TISSUES
VERTEBRATES
560152* - Radiation Effects on Animals- Animals